Chromatography

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary... | Contact experts in Chromatography to get answers

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Questions related to Chromatography

About pesticide recovery in poultry feed i got 30% recovery how do get 100 %recovery?

but i need 100 % recovery how do possible i try in high pressure chromatography only

22 November 2016 1,033 3 View

Which are the continuous bioprocess chromatography systems available in market currently ?

Like Akta PCC-GE, Cadence BioSMB-Pall, BioSC-Novasep , Octave -semba. Other than above system which are the other manufacturer ?

19 October 2016 3,170 1 View

Different models lumped mass transfer coefficient in chromatography?

I have a question related with the determination of the mass transfer coefficient for the transport-dispersive model in chromatography. According to what I read in the book of Guiochon...

17 October 2016 7,818 1 View

Can anybody suggest me a software that does GC-FID retention time alignment?

I am looking for a software that does automated retention time allignment of GCFID data. Can anybody suggest?

14 October 2016 9,260 3 View

If im using reverse phase HPLC using C-18 column, did i need to used RP-C18 TLC plates when performed thin layer chromatography too?

Hi i will be performed reverse-phase HPLC using C-18 column for identification and quantification of my interest compounds, did i need to use RP-18 plate too for TLC? Thanks.

12 October 2016 6,295 4 View

How can I prepare deactivated silica gel with 12%v/w water for chromatography?

The mobile phase is EtOAc-hexane (2:8)

11 October 2016 9,454 5 View

What could have happened to my spidroin@silk fibre protein (180-350kDa) after filtration with 0.2 um membrane? Not detected on the FPLC chromatogram?

Reasons??

08 October 2016 5,091 2 View

Can someone please tell me any protocol to check the protein profile of my test subject's serum sample via FPLC instrument?

I would need information about what column to use and if elution would be required or not.

03 October 2016 978 3 View

How i can separate hydroxymethylfurfural (HMF) from water solution?

I used ethylacetate but yield was very less please suggest me what solvent system i can use for extraction.

28 September 2016 254 3 View

Can silica gel blue be used for column chromatography or powdered silica gel. Which is appropriate?

Please I need an urgent response to this question. Thanks

20 September 2016 7,832 3 View

How to calculate chromaticity coordinate from PL data/graph?

I have PL data as well graph with three peaks at 416 nm, 480 nm and 520 nm. Using PL how can I calculate color matching function and chromaticity co-ordinate from my PL data/graph. I need solution...

19 September 2016 9,475 1 View

Data and methods in old Science papers?

Dear collagues, This is probably a very naive question but to date I haven't obtained a satisfactory answer. I often must study old papers. I recurrently find these old papers very reticent and...

12 September 2016 4,662 7 View

P Dioxane (1 4 dioxane) in Ethanol by GC-MS?

Dear All, Can anyone suggest me the conditions of GC-MS for P Dioxane in Ethanol. along with Column & parameters. I Need Quantitation in 10 ppb Maximum I have only MS no Headspace & Purge...

09 September 2016 9,673 1 View

Ion exchange chromatography?

I have performed gel filtration (superdex 75) and anion exchange chromatography for my protein. Solvent used for gel filtration is 20mM PB + 100mM nacl (ph 6.5) and for cation exchange, solvent A...

29 August 2016 3,382 12 View

What are the experiments that can be done to establish dimer formation of a protein in solution?

A protein is expected to form multimers in solution. How can I establish this fact? What experiments should I perform apart from Size exclusion chromatography and native PAGE?

28 August 2016 879 5 View

Can I use 2,4,6-trimethylpyridine as an internal standard in GC-MS-SIM instead of "2-acetyl-(13C methyl)-1-pyrroline" for 2AP estimation in rice?

Except the isotope labeling, what are the suitable standards for quantification of 2AP in GC-MS or GC-FID?

25 August 2016 4,093 3 View

Which is the best LC method of analyzing CMC-Na in a formulation?

Formulating a drug product that has to be filtered and wanted to know if there is an easier method to assess the amt of CMC-Na chromatographically.

22 August 2016 2,462 1 View

OpenChrom GCMS search libraries?

Hi I have just downloaded and installed this (rather complicated) program OpenChrom for OSX. I am using it to see chromatograms and spectra of GC-MS in different formats. I am, however, unable to...

09 August 2016 7,210 4 View

Does anyone have a protocol to make/pack their own ziptips?

I'm trying to a kinetic methyltransferase assay and would like to use ziptips as a means for quick RPLC, but I'll need hundreds of ziptips to generate good data and it just seems like a really...

02 August 2016 6,069 3 View

Any advice for TLC eluent of Nisin ?

Lately i've been researching Nisin in a microsphere system. I have a problem in methods to quantify nisin from the system. I've tried using UV spectrophotometry but it seems the polymer matrix...

02 August 2016 4,767 1 View

Schiff-base ligands and their complex are usually not stable against silica or aluminium oxide in chromatography .explain the reason ?

The design and synthesis of binuclear and poly nuclear transition metal complexes with macrocyclic Schiff base ligands.i m doing work on this topic

31 July 2016 7,763 3 View

Could anyone suggest the fragmentation pattern/product ions of spironolactone on LC-MS/MS (QQQ)?

The specific ions that I'm looking for are 341 and 107. Thank you.

24 July 2016 4,268 6 View

Can chromatography be used in in-vivo studies?

Is it feasible to use chromatography in in-vivo environment. I am looking for specific answers and the usefulness of Chromatography. I would appreciate any suggestions or comments

20 July 2016 4,966 3 View

Hi, what is the wavelength range required for flavonoid estimation using vanillin reagent?

am conducting flavonoid estimation using vanillin reagent. 0.2ml of sample + 1.8 ml dis. water + 4ml vanillin regant. can any one tel me the wavelength used to estimate this??

29 June 2016 9,975 3 View

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