Which procedure would be the most successful to determine glomalin in soil?

We have to determine easily-extractable glomalin (EEG) and total glomalin (TG) from soil samples, but we have found various contradicting methods and calculations. Is there a trusted method that...

20 October 2020 3,051 3 View

What kind of qualitative method to use?

Dear research colleagues, I am currently working on a qualitative research and would like to ask your opinion. I am not sure which qualitative method to use. We conduct semi-structured interviews...

14 April 2020 5,777 17 View

How can teacher education reflect the pedagogical ideal of democratic schooling based on dialogue?

04 March 2020 1,801 16 View

Does one of my research questions allow pooling of 2 intervention groups in a one-way ANCOVA?

Hey guys! Looking for some statistical advice to anticipate review response. I have done an intervention with 3 groups, 2 of which are in the intervention and 1 that is control. The 2 intervention...

06 September 2019 5,316 3 View

Thermal conductivity of Aluminium 5083 and 5754 alloys with temperature?

04 March 2019 6,653 6 View

GW170817: What is the relativistic mass of neutron stars in a binary system just before merge?

From the signal frequency received by LIGO gravitational wave antennas that is interpreted as originating from a binary neutron star system, we calculate orbital speed of merging components at...

04 February 2018 1,190 42 View

Should the electron rest mass be looked at as an elementary quantity?

07 October 2017 1,971 22 View

Does formation of elementary particles confirm Mach's principle?

23 September 2017 9,642 50 View

How to probe the local gravitational potential originating from remote masses of the universe?

Is the inertia of accelerating masses to be interpreted as due to interaction with the local gravitational potential originating from remote masses of the universe? A gravitational drag experiment...

21 September 2017 8,743 32 View

How to make an input file for CASSCF single point energy calculation for a molecule at triplet state (T1) in gaussian 09?

I want to calculate the single point energy of a molecule in the T1 state using CASSCF. Can anyone post a test file for the above mentioned calculation? The molecule is more stable at S0.

20 June 2017 8,632 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View