Dear Sheikh,

You can obtain a profile of your sample by separating it on a gel filtration column, e.g. superdex or similar. These are available as small volume analytical columns, which allow fast runs to check purity, oligomerization etc. However, the resolution of gel filtration is rather poor compared to e.g. SDS-PAGE - is there a particular reason you don't consider this instead? 

Hope this helps,

Silas

Hello Silas, 

I want to check the concentration of different proteins in the serum samples of my test subjects. i do not want to purify the proteins. I have FPLC instrument and no HPLC. Can you suggest me what best use I can make this instrument of?

If you want to obtain a protein profile and the only instrument you have available is FPLC then you should try with a ion exchange or hydrophobic column. Yes, of course you will need to elute, that's the point of doing liquid chromatography. Please seek expert advice from someone that can train you with this technique.

FPLC will not allow you to quantify individual proteins in a complex mixture (or in any situation), you can only obtain a protein profile for comparative purpose.

Finally, I agree with Silas, FPLC is very poor resolution for obtaining any useful information about protein profile, SDS-PAGE is much better - and easier - and it is available in any lab dealing with protein preps; if you find an FPLC, you should be able to find some kind of gel box and power supply as well, even old ones will do.