Chromatography

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary... | Contact experts in Chromatography to get answers

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Questions related to Chromatography

How to calculate surface charge of a protein with unknown structure?

I want to know surface charge of a protein of 1290 aa long for ion exchange chromatography isolation. Kindly suggest me how to calculate it.

09 October 2017 4,794 4 View

Which indicator other than bromocresol green can be utilized for organic acid detection in thin layer chromatography? Plz share protocol if known?

I want to detect organic anions qualitatively using TLC. What mobile phase (composition) and indicator solution (other than bromocresol green) can I use to do so?

05 October 2017 3,789 3 View

Which type of containers are appropriate for storing a chromatography resin?

Can I store the resin in a glass beaker after using it as sometimes I stored the resin long time with 20% ethanol in chromatography column (glass). Is glass bottle or beaker deteriorate the...

04 October 2017 7,541 3 View

What is the maximum sample capacity for HiLoad 26/600 Superdex 75 SEC?

I read in the manual that the maximum volume is 13ml, but it does not state the maximum protein concentration. Does anyone know it? Thanks.

29 September 2017 9,469 1 View

Can we use amino column instead of aminex column for the determination of organic acids? Though both of them are ion exchange chromatography?

Both the column has given the same answers.

14 September 2017 5,852 3 View

TBA contamination, anyone has a cure to get it out of your UPLC?

Hi, I have been doing some ion pairing chromatography, with TBA (tributylamine). Seems now that when i measure in ESI+ there is a huge background of TBA (m/z 186.2) and i can't get it out of the...

06 September 2017 3,832 6 View

LC MS TOF/Do you think that lc ms tof can now replace lc ms ms instruments for routine pesticide residue analysis?

Dear All LC MS TOF can carry out non target analysis, in addition the isotopic profile for the obtained data make the identification of these compounds more easier and reduce false positive...

03 September 2017 8,694 23 View

Possible techniques to measure the pore size of an electrospun mesh?

The most commonly reported technique in the literature to measure pore size is by SEM. However, I am doubtful regarding the accuracy of this technique for various reasons, the primary one being,...

18 August 2017 7,824 5 View

Is it possible to separate clipped forms of glycoproteins by chromatography?

Need some suggestion for separation of clipped forms of glycoprotein by chromatography.

08 August 2017 8,355 2 View

What is the terminology of the chromatogarphy column made by yourself/myself?

Dear, Please tell me the terminology of the chromatography column which is made manually. I mean, I have to purify of protein in larger scale. I will make a chromatography column by myself, but I...

08 August 2017 7,265 7 View

Hello Everyone

what is difference between size exclusion chromatography, fluroscence size exclusion chromatography (FSEC) and size exclusion chromatography-multi angle light scattering (SEC-MALS)? I want to...

31 July 2017 8,512 3 View

In silica gel column, when eluting a compound, I obtain much previous compounds (which were previously eluted out) along with the new compound. How?

I am running ethyl acetate extract on silica gel column using hexane and ethyl acetate as mobile phase ( by stepwise-gradient increase of polarity). When the compound elutes, I run in the same...

28 July 2017 1,101 7 View

Choline analysis by Ion Chromatography

Our group is trying to perform a chemical analysis of choline (as choline bitartrate) in clean water samples (containing only this chemical). We are using a Dionex ICS-5000 + DC Detector, with a...

28 July 2017 9,006 3 View

Repairing a compressed 3.2/300 column

I have a near brand-new 3.2/300 superdex column with compressed resin; plus a bunch of old ones in different states of decay. I would like to fill the gap with fresh resin, but these columns were...

21 June 2017 1,136 2 View

Protein precipitation issue in elution of Anion exchange chromatography

Dear all, Anyone can suggest me regarding a precipitation issue in elution of Anion Exchange chromatography, After elution thread like particles are forming in elution pool. We are using PB...

20 June 2017 5,232 9 View

Detect aninline

Hi. I want to use Thin-layer chromatography to determine aniline . But i can not find how i can do it (method, Solvent system, Composition)? How to detect the aniline line?

15 June 2017 5,995 3 View

How to extend column life with 100% aqueous mobile phase.

Hi Everyone, I work in a lab for a pharmaceutical company. The brand new column we purchased can only last 16 injections (a week) shown by lowered peak separation index and badly shaped peaks....

14 June 2017 6,960 8 View

I need suggestions for purification methods of Rtx family toxins

Could someone suggest me any general purification method of Rtx toxin from liquid culture,As I am trying to purify one of the Rtx toxins through Hydrophobic Chromatography but I am getting a high...

10 June 2017 8,125 5 View

Does PEG contamination interfere with a Bradford and/or BCA assay?

I have recently purified some IgG via affinity chromatography. As a result the elutions from my column were pretty dilute. I concentrated them by using PEG to pull out the excess water but I...

07 June 2017 2,324 3 View

I need to detect same compound using HPLC in different sample. Should I construct different calibration curve for each and every sample I studied?

I need to detect styrene monomer from different food sample

04 June 2017 3,784 10 View

Can i predict the unknown compound just by its retention time in HPLC?

I have injected crude solvent extract from mycelial cells in C18 column.

01 June 2017 3,516 13 View

Can we reuse electrolyte during ORR and OER electroclatalytic measurements?

Hi everyone, I am using 0.1 M KOH aw well as 1 M NaOH can we reuse the same electrolyte for new sample in RRDE experiment.

25 May 2017 4,392 2 View

What does it mean when the same spots are developped in both reversed phase and normal phase TLC

Normal phase system is methylene chloride:methanol 9.5:0.5 Reversed phase system is methanol:water 9:1

23 May 2017 9,634 5 View

Query about purification of fusion protein by Ni NTA chromatography ?

I am facing problem during purification of cytosolic fusion protein, by using Qiagen kit spin column (binding capacity 300 ug of protein) 80% protein is coming out in flow through so less yield is...

11 May 2017 9,303 7 View

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