Chromatography

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary... | Contact experts in Chromatography to get answers

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Questions related to Chromatography

How to activate sephadex G-50 for affinity chromatography?

What is the general protocol to activate sephadex G-50 for affiinity chromatography of lecitn?

15 May 2018 4,097 5 View

Chromatography with superdex G75. how much protein?

Dear colleagues, I am trying to purify a app.11 KDa protein (Enzyme) with chromatography method. I am going to use superdex G75 resin with a column length of 60 cm and 120ml of resin. but i do...

06 May 2018 8,550 7 View

I'm using High performance anion exchange chromatography to separate & identify different sugars in plant. Could anybody help me resolving a problem?

System Condition: The analysis was performed on a Metrohm IC system. Separation of all standard mixture was achieved with the help of Hamilton RCX-30 column. An isocratic system was employed...

24 April 2018 3,838 5 View

What is Bind-elute and flow through mode?

Protein PI 8.6 after Ni NTA affinity it is passed through Desalting column, desalting column equilibrated with pH 8 Tris eluted with tris then IEX (DEAE), IEX equilibrated with buffer pH 8 Tris...

13 April 2018 2,623 6 View

Can isoelectric points of my protein affect nickel affinity chromatography?

When purifying my his-tagged protein much of it was eluting at only 50mM imidazole. The protein was binding to the charged beads as shown by samples from the beads and supernatant after incubation...

31 March 2018 5,240 4 View

How to determine the absolute configuration with circular dichromic chromatography?

Recently, i have met a problem that i want to confirm the absolute configuration of a compound. The compound dose not protein, just a simple one that contains one chiral center. I want to know if...

14 March 2018 8,199 2 View

Dear, researches, How can I use sepharose Cl6B already equilibred on chromatography ion exchange ?

Hello, i would like to use chromatographie for protein purifications and we dont know how to use sepharose Cl6B who alrealy equilibrated with buffer unknown.

06 March 2018 2,219 4 View

Sephedax HL-20 has become a block not powder in the purchase box.. How do I treat it?

chromatography, sephedax.

23 February 2018 3,017 2 View

What about the Vion from waters?

Hi all, I am really excited as I recently found the Vion IMS QTof from waters. It was released in 2015, so it is quite new. Has someone worked with the Vion? Any advantage/disadvantage in...

28 January 2018 2,781 2 View

Why hiv gp140 trimers in chromatography shown a peak between ferritin and thyroglobulin references?

I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.

22 January 2018 9,855 2 View

Does anyone know, how to prepare protein-based electrochemical biosensor from bacteria?

We have transgenic E. coli and we need to isolated and purified the specific protein. We used membrane filtres, however it didn't work as we expected. We don't want to use chromatography for...

19 January 2018 9,825 2 View

I would like to know about affinity chromatography?

i have a presentation on it

17 January 2018 1,929 3 View

What tool can analyze a list of deferentially expressed genes, and return potentially affected pathways?

Hi all, Following a single cell RNAseq experiment, I found two cell populations of interest with starkly different transcriptomes. I would like to take the top 100 deferentially expressed genes...

11 January 2018 3,736 5 View

Common column for HPLC and Ion exchange chromatography ?

I am looking for a chromatography column which can be used for both separation and analysis of protein samples . I want to use it for HPLC and IEC.

03 January 2018 4,332 4 View

Can anyone suggest best possible resin for the Human Beta-2-Microglobulin purification from an albumin (60% - 80%) containing sample?

I have a feed containing B2M, albumin and antibody fragments from which i want to purify B2M as my sole target protein using Chromatography resin in one or two steps. If anyone has any good...

13 December 2017 9,460 3 View

Will the presence of Imidazole(500mM) influence the enzymatic activity of Proteinase 3?

We obtain a recombinant protein from chromatography in 500mM imidazole. We need to cleave this product with Proteinase3. Is Imidazole going to influence/hinder the enzymatic activity of Proteinase3?

11 December 2017 4,458 2 View

Hi everyone, What kind of chromatography is better to use SEC or HPLC to analyse polymers with molecular weight of about 6000Da?

Chromatography, SEC or HPLC

02 December 2017 3,140 11 View

How can I add more than 5 columns to an Akta Pure?

Akta Pure limits the number of columns per system to 5 due to the C-valve. What is the best way to add more columns to the system? I've heard of people using the loop valves to double the columns...

22 November 2017 6,287 3 View

How to identify peptides bound to protein?

Hello, I would like to identify peptides bound to the particular protein by MS. Do you know how to separate these peptides from protein using method allowing further analysis by MS? This protein...

13 November 2017 5,271 4 View

During LDHA purification using Ni2+-NTA chromatography, impurities were still found in protein, how can i improve?

For LDHA enzyme purification using Ni2+-NTA chromatography, the following buffers were used: (1) Ni binding buffer with 20mM imidazole (2)Ni washing buffer with 100mM imidazole. However,...

30 October 2017 7,829 7 View

How to isolate the fractions of gamma irradiated sodium alginate using the column chromatography technique?

I am using the sephadex as stationery phase and didtil water as moblie phasem in column chromatography. So my question is how to isolate the fractions obtained via column chromatography for the...

25 October 2017 9,610 2 View

Is there any detriment occur if a column equilibrate faster than the recommended flow rate?

Suppose the column packing flow rate is 30 cm/hr and the recommended sample run flow rate is 15 cm/hr; the resin pressure is maximum 0.02 Mpa. As the size exclusion chromatography requires longer...

18 October 2017 611 4 View

Why mean responses are used to define surface response?

in the second (optimisation) step of application of design of experiment, we tried to set a model to define most influential factors"we call too surface response methodology" , in parallel, this...

16 October 2017 3,587 2 View

How to increase recovery in ion exchange chromatography?

11 October 2017 1,366 2 View

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