Chromatography

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary... | Contact experts in Chromatography to get answers

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Questions related to Chromatography

Is Terpene Analysis possible by using HPLC?

Citrenellol, carvacrol etc.

12 December 2013 8,558 8 View

Could anyone give me a suggestion regarding saponin isolation?

Actually I am currently working on saponin isolation but having very closed spot of four saponins in TLC and I have tried C18 column in preparative HPLC but it didn't get separated. After that we...

12 December 2013 1,502 9 View

Detection of traces of ethyl formate in ethyl acetate?

Does anyone have experience in the detection of trace-levels of ethyl formate in ethyl acetate?

12 December 2013 9,297 3 View

What are the disadvantages of using simple calibration curve in quantitative analysis by GC/FID?

What are the disadvantages of using simple calibration curve (i.e. plotting standard concentrations against peak area for each batch of sample run) compared to external or internal standard...

12 December 2013 3,499 23 View

How can I prepare a 3% defatted Bovine Serum Albumin solution?

Solution preparation.

12 December 2013 7,262 1 View

What are the exact definitions of "ultra inert" and "deactivated"?

What do terms exactly mean: "Ultra inert" in case of liners, columns, and "deactivated" in case of glass wool? What is the process to make the glass to ultra inert glass?

11 November 2013 7,256 2 View

How can I extract proteins from plant cells?

11 November 2013 7,417 0 View

Has anyone performed HPLC on extracts from Hopea pierrei wood?

Has anyone performed HPLC on extracts from Hopea pierrei? Do you have a list of flavonoids? What columns to use?

11 November 2013 6,962 2 View

Why is smear formed when during SDS PAGE?

11 November 2013 2,725 4 View

Since the volume is equal,why peaks are not equal

the detector of gc is FID

11 November 2013 9,905 5 View

Which is the best way for determining naphthalene concentration in water?

I would like to determine naphthalene concentration in water for a wastewater treatment project. Would it be possible to use chromatography technique or would you suggest something else?

10 October 2013 8,737 5 View

Which protocol is better to use for aminoacid extraction?

For the extraction of aminoacids from complex matrices, (i.e. coffee) intended for chromatographic detection, and having a hydrophobic C18 column, which solvents and which protocol among the ones...

10 October 2013 9,984 5 View

Is it possible to measure compounds dissolved in water using GC-MS?

I am working with a biofuel cell for the production of formic acid and methanol. I ran into a problem analysing the products formed. I found in the literature that methanol, formic acid and...

10 October 2013 5,120 16 View

What is the purpose of ramping while performing GC?

I'm learning GC recently and came across many analyses performing ramping instead of providing a constant temperature right from the start. What is the purpose and advantage of ramping while...

10 October 2013 8,519 5 View

Do you have an analysis method by GC-PDHID of hydrogen cyanide?

I would analyze hydrogen cyanide and ammonia by GC-PDHID but I don't see the peaks of these compounds on the chromatogram.

10 October 2013 5,077 2 View

What settings are recommended to analyze CO2 and H2 with TCD using Carbo Sieve S-II column?

CO2 is not being detected with N2 as carrier gas. Will Argon work to detect it? What are the flow rate and temperature settings recommended to the above analysis?

10 October 2013 6,605 1 View

How to minimize phthalates contamination step by step during phthalates analysis?

Chromatographic interference from phthalates contamination.

10 October 2013 8,524 8 View

What is the best method to eliminate spots of compounds on TLC sheets which were used in controlling the reaction?

TLC sheets are used for controlling the chemical reaction ,,,spots of compounds on TLC sheets make it useless again. What methods are used for purification these sheets?

10 October 2013 352 4 View

Can someone educate me on how best to treat a data report generated from GC/MS analysis?

Modern GC/MS instruments normally have a library of compounds which were compared with your sample when it ran. When data are generated it is observed that many compounds are suggested for a...

10 October 2013 1,214 2 View

What extraction protocol can minimize contamination of plant extracts by chlorophyll?

Other than sephadex LH 20 and Carbon.

26 September 2013 4,685 9 View

Can anybody share a Dimethyl amine content method in drug substances at genotoxic level?

Most of the methods on the net are on ion chromatography. It does not give reproducible results, some are GC methods but don't work. If you know any authentic method please share it.

09 September 2013 3,871 1 View

What method can I use to obtain flavonoid and phytosterol rich fractions from plants?

I would like to obtain these and quantitatively compare to obtain data for further studies.

09 September 2013 7,424 7 View

Which is a better medthod for determining the vitamin A content of bananas? Thin-layer chromatography or Column chromatography?

Vitamin A content of bananas

09 September 2013 8,236 1 View

How can I purify the silica gel that is used in column chromatography to be used again?

Silica gel is expensive and I want to know a cheap method for purifacation that I have used before.

09 September 2013 731 2 View

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