Chromatography

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary... | Contact experts in Chromatography to get answers

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Questions related to Chromatography

Hi. Can anyone suggest me the difference between CombiFlash, Flash chromatography?

What is CombiFlash chromatography?

28 February 2016 6,478 3 View

How diffirenet is it to make the gel-filtration chromatography graphs based on time or volume?

Hi, I have done some gel-filtration chromatography and now that I want to plot my results, I am not sure if it should be UV absorbtion per time (min) or UV absorption per volume? Does anybody have...

25 February 2016 2,282 3 View

How to isolate Imine by column chromatography?

I made Imines by oxidation of Amines I want to isolate them by using column chromatography. I used Activated neutral alumina oxide as given in reference papers but after after column when I check...

20 February 2016 4,121 2 View

Why analysing antibiotics the chromatographic peak are large and disform?

we are using the column Kinetex C18 column (2.6 x 150 mm i.d., 1.7 µm particle size.and changing the proportion of the mobile phase (acetonitrile and water) no big change was observed.

19 February 2016 874 5 View

Subunits of glycoprotein separation with wide pore HILIC comlumn

we have digested glycoform proteins (Subunits of protein) and tried to separate them by HILIC widepore column. Is anyone have a good chromatography to recommend?

18 February 2016 8,860 3 View

How to get rid of or identify a peak in a chromatogram for GC/C/IRMS?

We are using HS-SPME-GC/C-IRMS. I have three images attached to this question. The first one is of an instrument blank. There are no peaks present, which is what we want. When we do a fiber blank...

15 February 2016 3,037 3 View

Lumenfantrine analysis by HPLC as per USP ?

Hello Friends, I am working on Lumenfantrine analysis by HPLC as per USP method, but in USP 38 column temperature mentions as Column temperature: Beginning of column, 50°; end of column, 35°. This...

10 February 2016 1,256 4 View

How can I further fractionate bacterial conditioned media?

I am trying to identify an unknown molecule/peptide that is secreted by a bacteria and has a particular activity in my cells. I have narrowed it down to something that is less than 3kD. I have...

01 February 2016 990 4 View

Methods for improving purification yields of sticky proteins?

Does anyone know of a text book or handbook describing methods/strategies for improving purification yields of hydrophobic proteins, in particular, methods for improving the yield of protein...

01 February 2016 1,197 1 View

Does anyone know why my 1N NaOH solutions have a high absorbance at 280nm during chromatography runs?

I sterile filter the solution using a 0.22 PES Filter. The absorbance increases as the conductivity increases. Its about 1500 mAU per ~180 mS/ml.

14 December 2015 3,791 6 View

Which elctrolyte can be used in electrocoagulation treatment of wastewater?

I am working electrocoagualtion treatment of wastewater and want to use electrolyte, for the adjustment of conductivity of solution and enhance the reduction of pollutants.Electrolyte should be...

07 July 2015 8,246 20 View

Do I need an accurate mass instrument for global (untargeted) metabolomics?

I am going to do VOCs analysis using Shimadzu GCMS (it is not an accurate mass instrument). I just want to know if I will have problems with compound identification later by MSMS analysis.

06 November 2014 2,611 3 View

Can anyone help with problem regarding overlapping peaks of sodium and ammonium during standardization?

We are getting overlapping peak of sodium and ammonium during standardization in ion chromatography. We use 1PPM 3PPM 5PPM and 10PPM as standard. The problem is mostly happening in case of 3,5,10...

23 July 2014 1,524 12 View

What is the mechanism of densitometric scanning in TLC?

What is the difference between scanning a TLC plate at 550 nm (Visible), 254 nm (UV) and 366 nm (fluorescent)?

22 July 2014 6,110 7 View

Can anyone help with bisphenol A in LC TOF MS?

I have tried detection of BPA using LC-TOF-MS in both negative and positive mode with no any peaks. Any suggestions? Thanks

13 July 2014 7,484 2 View

Does anyone have the optimized protocol for GLC analysis of short chain fatty acids in a broth medium?

I want to analyze acetic, propionic and butyric acid in MRS broth (supplimented with inulin) fermented by probiotic bacteria. There are similar kinds of studies suggesting different types of...

04 July 2014 8,809 3 View

How can you calculate the concentration of a compound in a fraction by HPLC ?

I fractionated an extract of plant and I took 15mg in 20ml solvent of a fraction and injected to HPLC, got a sharp peak with good resolution, and I injected a standard compound of concentration...

21 March 2014 2,507 37 View

Is it possible to pack a gel filtration column by Akta Prime machine pressure and flow rate control?

Whether pressure control and flow rate by AkTA Prime machine are sufficient for column packing.

04 March 2014 5,946 2 View

How can I get a linear calibration curve for CO2 by GC-TCD?

Several calibration points (from 1% (v/v) to 30% (v/v)) of CO2 in the air are measured, but the response of the detector is non-linear. I'm using a Focus GC (Thermo Scientific Instrument)...

16 February 2014 2,882 2 View

Can anyone help me out for C-reactive protein isolation protocol?

I want to isolate CRP from human serum. What is the best procedure to isolate it? With which compound does it bind most efficiently so that we can isolate it using affinity chromatography?

01 January 2014 383 1 View

HPLC or GC?

I want to detect the alcohols in my sample. So HPLC and GC (FID and TCD -Detector) which I am about to use consists of C18 column, and UV detector (For HPLC). The GC is equipped with agilent...

01 January 2014 2,459 13 View

Span 60 and Tween 60 analysis?

I was wondering has anyone ever analysed span 60 (sorbitan monostearate) and tween 60 (polysorbate 60) quantitatively using instrumentation? I have a LC - UV, LC-DAD, GC-FID, and LC=ELSD available...

01 January 2014 4,383 2 View

Can anyone kindly help me with the standard ratio of solvent used for crude extraction of dried plant material?

I will not mind if a supporting reference can bemade available also.

01 January 2014 4,090 7 View

Anybody have the procedure for TLC to test Avermectin?

I want to do TLC for Avermectin assay but I do not know how to do it.

01 January 2014 2,883 2 View

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