Hello,
I try to establish a 107 a assay unfortunately without success. I used human PBMCs and cocultured them with several cancer cells (with and without developed drugs). The flow results looked like a full degranulation even when PBMCs were cultivated alone. PBMCs were isolated with standard procedures. Does anyone have an established 107a assay protocoll which could improve my results?
Michael Schmohl
Did you ever try a different aCD107 antibody for staining or modify the washing procedure to lower unspecific binding
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