I have done this Cips experiment twice using classic 7 compounds in paper，only showing morphology change of MEF and cloning，no Oct4 activation，no Nanog activation，only showing the reactivation of early reprogramming gene，like cdh1，sall4，and sox2 in paper. And when I picked the clone on feeder cells  on D40 around,I found these clones hard to dissociate to single cell by trypsin,and no viable subclones! I wonder has anyone successfully repeated this experiment?

Lets share it!