Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Different antibodies show signal at same MW in SDS-PAGE, what is wrong?

16 February 2021 2,857 9 View

The modern system of international relations: unipolar or multipolar world?

Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?

01 January 2021 7,026 2 View

Mie scattering of a Gaussian with curved wavefront by a sphere?

Can anyone please suggest some references? I would like to simulate scattering of a fundamental mode of a fiber by a microsphere. I am only familiar with a plane wave case, but in case of a curved...

01 January 2021 1,440 3 View

Blood and tissue DNeasy mini kit 260/230 contamination?

I've been trying to extract DNA from rat liver tissue using Blood and tissue DNeasy mini kit. I followed handbook procedure and all of my samples had low 260/230 (

21 December 2020 7,100 3 View

Student's t-test or DeSeq2 for RNA-Seq data analysis?

Hi everybody, I am analysing RNA-Seq data of patient samples. One group of samples has a certain monoallelic deletion, the other group 2 copies of the wildtype gene. Now, I want to compare the...

28 November 2020 5,977 3 View

Cre-loxp system does not work (lines #017455 and #017320, JAX). Why?

We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse...

16 November 2020 8,701 1 View

Why after cloning recombinant pET in E. coli DH5a I get a very low plasmid yield after extraction?

I chemically transformed a ligation product (pET28a and my gene of 3051 bp) into E coli DH5alpha competent cells. I plated 200 and 800 ml of my transformation on plates of LB agar Kan (25 mg/ml)....

13 September 2020 4,421 3 View

Good markers to distinguish D1R/D2R striatal medium spiny neurons?

Hi! I want to distinguish D1R and D2R subpopulations of medium spiny neurons in striatum in immunofluorescence. Most of the papers I found use transgenic EGFP mice targeting this receptors, but I...

31 August 2020 3,863 2 View

does anyone have the user manual of the geostrain program?

Hello, I am looking for the user manual of the geostrain software, I have downloaded the software but the user manual is not found. I will be very grateful for your help Cheers

14 June 2020 4,169 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View