Hi Researchgate folks,
Our lab has mouse FFPE tissue which has cells that express a TdTomato reporter as well has a FAM labeled drug as we routinely use to test the delivery of the drug to the target cells. For some downstream spatial transcriptomics work we need to find a way to quench these fluorophores in FFPE sections prior to sequencing. Does anyone have any experience with quenching these fluorescent molecules/proteins?
Thanks!