How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

21 April 2019 281 4 View

Films prepared by combination of Hypromellose E15 and E5 polymer with 20% of plasticizer (PEG 400) w.r.t dry polymer using water results in brittle ?

This question is related to soluble film formulation.

04 September 2018 3,086 1 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

05 March 2018 4,842 6 View

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only?

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only. Please help..

22 January 2018 992 4 View

I wanted to see if all the morphs of nematodes are same species or not by molecular sequencing, how to proceed doing it?

20 March 2017 5,885 3 View

What is the function of acetic acid and methanol in staining-destaining of sds page?

function of acetic acid and methanol in staining-destaining of sds page

17 February 2017 3,392 4 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

02 November 2016 1,753 3 View

How to reduce size of a void in a metal from micron to nanometer range ??

I want to know if there is a way in which the void of the material inside the metal can be divided from micron size to nanometer size by splitting the voids into discrete voids of reduced size...

03 October 2016 6,811 3 View

How is the molecular phylogenetic analysis of foraminifera beneficial for taxonomic studies?

I would like to know the actual role of rDNA and protein sequences in evolutionary relationships of foraminifera.

23 August 2016 9,210 2 View

Has anyone implemented Self Organizing (Adaptive) Fuzzy controller?

I have read a lot theory about Self Organizing Fuzzy control from Internet. But I am still not completely able to understand its theory and implementation.I have attached one of the best document...

02 June 2016 2,438 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View