We have been culturing MSCs from knee bone chips in which the MSCs migrate out into the culture flask. These have been taken from someone else in our group and resurrected from frozen (P3-4). However, when we have cultured these cells from frozen we seem to be getting a cellular contamination that is rapidly proliferating (can generate 12 million cells in 5 days, T175) that takes over the flask that wasn't seen in culture with the other group. Can anyone help with identification of what these cells might be and suggest any flow markers that could show up?

Note: magnification on image is x20 and cells have been seeded at very low confluency to show morphology.

Thanks for your help.

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