We have been culturing MSCs from knee bone chips in which the MSCs migrate out into the culture flask. These have been taken from someone else in our group and resurrected from frozen (P3-4). However, when we have cultured these cells from frozen we seem to be getting a cellular contamination that is rapidly proliferating (can generate 12 million cells in 5 days, T175) that takes over the flask that wasn't seen in culture with the other group. Can anyone help with identification of what these cells might be and suggest any flow markers that could show up?
Note: magnification on image is x20 and cells have been seeded at very low confluency to show morphology.
Thanks for your help.
Hi Rebe
From your image these cells show pleomorphism and increased nucleocytoplasmic ratio with very high mitosis and proliferation rate as you reported that you really get 12 millions cells from one frozen vial within 5 days.
For me it is a highly proliferative malignant cells.
If you culture on coverslips or microscopic slide and stain them with the rotein pathology stain H&E you can see more detais and the nuclear hyperchromatism and atypia.
at this level on this image it is difficult to know the exact type of these cells.
Morphology is a very inaccurate way of figuring out what these cells are. You should perform STR profiling. If it's not available at your own institution, it is widely available at ATCC and at a number of companies.
Hi Rebecca
The cells look in a state of high mitosis due to cancerous transformation. you should use specific marker for the osteocyte cancer.
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