02 February 2020 2 8K Report

I guess I'm not understanding the concept well. If I treated cells (5000/well) with a range of doses from 6nM to 92nM (and this is an MTT assay and I've used Prism8), why is the IC50 only .9nM? I guess I'm not reconciling what I'm putting into the assay and what prism spits out. Are the values from Prism in the same units as my initial concentration? I also log transformed the values so Idk if that has anything to do with it.

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