What is a quick and dirty way to calculate dose response for a cancer drug on a cell line?

More Sri Harsha Meghadri's questions See All

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

I have calcined my catalysts without flowing air from the compressed air cylinder will there be any problem with my reaction if i use that catalysts?

FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion

25 July 2024 2,486 2 View

What is your opinion on secondary datasets in data analysis?

I am trying to write a detailed article Secondary Data Methods

20 May 2024 2,109 3 View

Can I apply two-step custer analysis of continuous variables for defining cases and controls?

There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...

14 May 2024 7,717 2 View

How do you decide which data visualization techniques to use for presenting your findings?

Les say you have a data that is has more noise and now you will need to decide which data analysis technique will be more useful and how do you start data visualization.

09 May 2024 8,935 3 View

How to develop a flat panel display glasses ?

Dear friends, I am looking to develop a new project for the applications of displays using float glass. How and what process is needed to develop such flat panel displays what are the ideas and...

09 May 2024 3,233 0 View

Regarding methanol to dimethyl ether kinetics on hzsm-5 ?

Hlo I am trying to get kinetics of methanol to dimethyl ether kinetics on hzsm-5 si/al = 40 catalysts at 160 deg c I was unable to reach zeroth order kinetic regime. I am doubting that the...

05 March 2024 1,930 0 View

How do we control pin holes in the coated thinfilms by using large area magnetron sputtering techniques?

Dear ResearchGate colleagues, professors, and scientists, I'm encountering challenges with coated thin films produced using a sputtering system. Following treatment through tempering at 650...

11 February 2024 3,585 4 View

Why does my Gold nanoparticle change colour over time without being exposed to any external agents?

I have synthesized gold nanoparticles that show UV Vis peak at 522nm and were bright pink immediately after synthesis. Over a span of 24hrs, the Solution shows visible colour change from pink to...

07 February 2024 10,125 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Amit Kulkarni

LDH assay and use of xCELLigence (need instrument for it)

Steingrimur Stefansson

Count live cells using Trypan blue exclusion with a hemocytometer.

Sri Harsha Meghadri

Trypan blue exclusion is what I am doing now. But the sample size is quite large, 2x96 well Plates ;) so sitting with trypan blue is not fun.

Hey. No pain, No gain.

Load your ipod with your favorite music, park yourself in a comfortable chair and count those suckers.

Zhen Dong

You can try CCK8, a more convenient way to detect cell viability.