I've done RT-PCR for TP53 gene and the results showed an increase of the gene expression comparing to its normal match and as known TP53 is a tumor suppressor gene which should be down regulated in colorectal cancer!
Yes, it's possible. Approximately half of all colorectal cancers show p53 (TP53) gene mutations, with higher frequencies observed in distal colon and rectal tumors and lower frequencies in proximal tumors. Therefore, you may need to do the sequence of TP53 before you have a conclusion.
Yes. Once p53 has oncogenic mutations, such as R175H in human or R172H in mouse, it gains oncogenic functions different from those of wild type p53.
You may read some review papers, such as "Mutant p53: an oncogenic transcription factor (Oncogene,2007) and Mutant p53: one name, many proteins (G&D, 2012) ".
Hello
I think that before doing any conclusion, it is important to consider several parameters:
i) first there are at least 8 TP53 RNA with different exons: therefore depending on the primers used for your analysis, you have targeted either some or all TP53 RNA
Ii) what tissue did you used ? Cell lines ? Tumour tissue ? What is the heterogeneity of the sample that has been analyzed ? if it is tumor tissue, it could be very heterogenous.
Iii) what do you call matched tissue ? Is it the same tissue type ?
best
Pr Soussi
please let me know which exon these primer amplify
If the tumor tissue is heterogenous (les tha 50-60%), it is difficult to say if the accumulation is due to the tumoral cell, the strroma, the infiltrating lymphocytes, etc etc. What is the purpose of your study ?
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