You should explain the aim of the study. Will you assess cytotoxicity of new compounds or mayby medical device?
I suggest to look for some information in the following publications:
1) "False" cytotoxicity of ions-adsorbing hydroxyapatite - Corrected method of cytotoxicity evaluation for ceramics of high specific surface area. Mater Sci Eng C Mater Biol Appl. 2016
Klimek K, Belcarz A, Pazik R, Sobierajska P, Han T, Wiglusz RJ, Ginalska G.
In this publication, the procedure of cytotoxicity assessment for biomedical materials was described with details.
2) Synthesis, antibacterial and antiproliferative potential of some new 1-pyridinecarbonyl-4-substituted thiosemicarbazide derivatives. Medicinal Chemistry Research 2016.
Pitucha M, Woś M, Miazga-Karska M, Klimek K, Mirosław B, Pachuta-Stec A, Gładysz A, Ginalska G.
This manuscript presented the cytotoxicity evaluation of novel compounds.
Try using WST-1 cell viability dye from Roche and follow manufacturer's protocol
Thanks Katarzyna Klimek .. i am looking protocol for novel compounds (small molecules).
Dear Jayaprakash Narayana Kolla,
I suggest you read this article Medicinal Chemistry Research 2016. Best regards
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