Can I use Fluorescein Diacetate (FAD) to check the viability of my plant cells after the H2O2 treatment?

Hi all, I am treating my 8 day old Arabidopsis thaliana saplings with different concentrations of H2O2 for variable time periods. After the treatment I want to check the viability of cells...

10 November 2019 2,346 2 View

Is it required to quantify cDNA concentration before going for semi-quantitative PCR of any particular gene?

To prepare cDNA, I took the equal concentrations of RNA of both control and experiment samples. The volume of control and experiment samples during cDNA preparation was kept equal. Do I need to...

02 March 2017 9,756 6 View

How long overhang I can keep in my primer?

31 December 2016 153 7 View

Do I need to go for Dpn1 treatment?

07 August 2016 409 4 View

Why I'm getting extra protein bands with my specific protein band during Ni-NTA His-tagged protein purification?

I have expressed a protein of C. jejuni in E.coli M15 cells using pQE30 vector. My protein carries a N-terminal hexa his tag. I have purified my recombinant protein using Ni-NTA columns and I have...

07 August 2016 9,231 8 View

How can I grow two different cell lines in one plate and even they don't merge in each other?

11 December 2015 2,725 4 View

What is the accuracy of epitope prediction software's?

I have no idea how these epitope prediction software's do work but I think using them will be somehow beneficial rather than designing primers blindly for the peptide synthesis. Before I use any...

08 September 2015 4,037 1 View

Why does my plasmid get stuck in the agarose gel well?

Hi, I've cloned my GOI into TA cloning vector( pDrive) vector, after I did transformation into DH5 alpha I got good number of colonies.After this when I'm doing plasmid isolation using...

07 August 2015 4,458 7 View

Can someone please suggest me the name of any tool or program where I can match two sequences side by side?

I want to know the name of any tool or program which I can download easily.I want to align two sequences side by side and and want to see the base pairs which are matching and the gaps. Also it...

04 May 2015 9,332 3 View

Is there any way to count cell number using phase contrast image? Can I calculate percentage differentiation using phase contrast image?

I'm having phase contrast image data of my previous cell culture where I have to count number of differentiated Mesenchymal Stem cells among normal cell population. can anyone please suggest me...

02 March 2015 6,342 8 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View