Why different plasmid with same back bone shows difference in mammalian (HEK293T) transfection efficiency with same transfection agent?

Hi All. I am using 4 different plasmid, each encoding different scFv with similar back bone, The only difference between all the plasmid is nucleotide encoding for scFv seq (Antibody Seq)....

01 February 2019 2,036 12 View

Is it advisable to calculate IC50 when my drug concentration haven't reached IC:50 ?

08 September 2016 8,596 4 View

Why body of tumor fails to carry single mutation that first arise/ initiated in stem cell ?

We all know that CSC (Cancer stem cell) are germ line of tumor giving rise to body of tumor cell. Then why this body of tumor cell fails to carry single gene mutation or single gene alteration,...

05 June 2016 9,112 3 View

Which type of cell is more likely to become tumor ? in the contest of Doubling time and Life span ?

1) Which one of the following has increased risk of becoming tumor ? A) Cells with shortest doubling time (often divide) and shortest life span. B) Cells with longest doubling time (rarely divide)...

05 June 2016 4,577 1 View

What happen when suspension of malignant tumor is auto-grafted to the patient from whom tumor is isolated? will it form tumor mass ?

05 June 2016 1,817 6 View

Can cancer call die without morphological changes ? or Dead cancer cell can continue to have intact morphology ? Will it be called senescence

03 April 2016 4,243 10 View

Cancer or tumor can arise from normal cell without any genetic changes ?

Here You go .. I was looking for answer ,can tumor arise from normal cell without genetic or epigenetic changes .. Answer is YES .. Here you Go...

06 July 2015 9,421 0 View

How is stacking is formed on the DNA ladder assay when the genome of a cell is cleaved uniformly into 180 bp during apoptosis?

As we know well the genomic DNA is cleaved into nucleosomal sub units by CAD enzyme during Apoptosis .. If every part of genomic DNA is cleaved to 180bp (Nucleosomal sub unit) ,then how do we get...

08 September 2014 6,869 5 View

How do MAMP can make the immune system to discriminate between beneficial micro organism and pathogen ?

06 July 2014 5,706 8 View

Will ALL acute lymphoblastic leukemia T lymphocyte/B lymphocytes protect themselves from foreign substances (Microbes) in cancer patients?

06 July 2014 3,722 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View