Why different plasmid with same back bone shows difference in mammalian (HEK293T) transfection efficiency with same transfection agent?

Hi All. I am using 4 different plasmid, each encoding different scFv with similar back bone, The only difference between all the plasmid is nucleotide encoding for scFv seq (Antibody Seq)....

01 February 2019 2,036 12 View

Is it advisable to calculate IC50 when my drug concentration haven't reached IC:50 ?

08 September 2016 8,596 4 View

Why body of tumor fails to carry single mutation that first arise/ initiated in stem cell ?

We all know that CSC (Cancer stem cell) are germ line of tumor giving rise to body of tumor cell. Then why this body of tumor cell fails to carry single gene mutation or single gene alteration,...

05 June 2016 9,112 3 View

Is there any gene specific carcinogen ?

05 June 2016 5,537 4 View

What happen when suspension of malignant tumor is auto-grafted to the patient from whom tumor is isolated? will it form tumor mass ?

05 June 2016 1,817 6 View

Can cancer call die without morphological changes ? or Dead cancer cell can continue to have intact morphology ? Will it be called senescence

03 April 2016 4,243 10 View

Cancer or tumor can arise from normal cell without any genetic changes ?

Here You go .. I was looking for answer ,can tumor arise from normal cell without genetic or epigenetic changes .. Answer is YES .. Here you Go...

06 July 2015 9,421 0 View

How is stacking is formed on the DNA ladder assay when the genome of a cell is cleaved uniformly into 180 bp during apoptosis?

As we know well the genomic DNA is cleaved into nucleosomal sub units by CAD enzyme during Apoptosis .. If every part of genomic DNA is cleaved to 180bp (Nucleosomal sub unit) ,then how do we get...

08 September 2014 6,869 5 View

How do MAMP can make the immune system to discriminate between beneficial micro organism and pathogen ?

06 July 2014 5,706 8 View

Will ALL acute lymphoblastic leukemia T lymphocyte/B lymphocytes protect themselves from foreign substances (Microbes) in cancer patients?

06 July 2014 3,722 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View