I measure tumors in thigh (mouse). I usually use a vernier but i find it somehow subjective. Tumors are not always perfect round wich makes it difficult, is there something else that might be helpful that eliminates subjectiveness?
Yes, computerized NMR, CAT, PET, or US imaging devices (US probes to be used with an SmartPhone or tablet are offered for sale), include software to assess tumor volume and linear dimensions. The subject of its Sensitivity and Specificity, compared with Pathology results, is discussed, I guess, in specialized Radiology magazines and texts.
Regards, + Salud
Hi, Isaias. Using a caliper (vernier) is the fastest and cheepest way. To incease the precision, you could measure the three dimensions of the tumor and use formula V=0.5236(LWT) to calculate the volume (http://www.ncbi.nlm.nih.gov/pubmed/24682747). You could also wieght the tumors to assess their density. Computerized MRI would be the most precise method, but it is expensive and labor-consuming.
Krzysztof, I agree, IVIS could be added to the list. It is good for detection of metastases - and for having beautiful images for paper, but, in my opinion, is less precise than caliper in quantifying the tumor volume (it yields a 2D image and to asses the volume one would have to take at least 2 images from different angles). Also, eventhough it is not the case for Isaias, the IVIS it is not that helpful when working with abdominal tumors (I've used it for implanted ovarian tumors), because it is hard to distinguish between the primary tumor and the surrounding metastases, if they are close. Finally, if the mice you work with have black fur, it may interfere with the luminescence signal.
We use calipers purchased from Bel-Art
web site www.belart.com
catalogue number 124160001
I agree with Rashid. The caliper is a fastest and cheapest way. We also found not 100% matching tumor size in the IVIS.
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