Hello Sandip,

Dear, all you need to do is dehydrate your samples fully by incubating them at high temperature. After dehydration iscomplete crush them to make a powder form and dry the powder completely as any moisture will interfere in acquisition. Now load the powder in FTIR by mixing with equal amount of KBR (before that run only KBR as background control) and acquire it (52 scans per sample).

You will get peaks for which you can find the details about functional groups in database.

Hope it helps !

Thanks Ajay

But actually i want to know which compounds & its structure (Like mostly all secondary metabolites ) present in plant sample. Mostly  how much concentration  of particular  compound is their in plant. i'm interested in this analysis. I want to know powerful method so that i can perform that only. instead of doing any other.

can u suggest any thing regarding this 

Hi Sandip,

You can use NMR spectroscopy for that. Metabolomics by NMR. However, if you really want to analyze the extract from a completely new species, the method won't give you any information. If you have an authentic/standard sample, you can use it for comparison and identifying the components in your extract based on peak position (chemical shifts). The kind of experiments one need to carry out also depend on the complexity of the mixture that you want to study. Its not straight forward.  If your plant sample contains only one component in it and you are looking for a complete structure elucidation of it, then a combination of mass and NMR will help you solve the puzzle.

best regards,

Uday. 

Thanks Udaya,

Do you know where any institute where i can do NMR? Also tell me if they are providing training on NMR. That will be more useful for me.

Hi Sandip,

I myself work on NMR at CSIR-NCL Pune. Ours is a research organization. We wont provide any training. Frankly, training in NMR is not possible in general unless one does PhD in it or has spent a good amount of time working on various examples for a couple of years. You are always welcome to approach us.

regards,

Uday.