Which one is best theoritical model that takes account interfacial thermal resistance while predicting thermal conductivity fiber/polymer composites?

Hello Everyone, I want to know about best analytical model that consider the interfacial thermal resistance which predicting the thermal conductivity for fiber/matrix composites. Here, Best...

30 December 2020 6,375 2 View

Can anyone specify the reasons behind the different microorganism inhibitory patterns between aqueous and methanolic plant extract (disk diffusion) ?

I have prepared an aqueous extract and a methanolic extract of a plant. Using the disk diffusion method, the microorganism inhibitory pattern I am intended to observe. In several literature...

20 December 2020 9,830 11 View

Can i apply bell method to equilize the histogram in ImageJ, please let me know the way if that can be done ?

This question is regarding the use of imageJ, I want to apply the same effect to my image that can be done by the command "ret = IpHstEqualize(EQ_BELL)" in image pro premier. I have imageJ only...

01 November 2020 5,660 1 View

How can i annotate a eukaryotic genome (partial sequence ) ?

I want to annotate a eukaryotic partial genome sequence. Length around ~700 bp. Is it possible using BioNumerics Or Genome Assembly! If Yes,then how? Any suggestions will highly be...

29 October 2020 5,527 14 View

How to choose solid lipid to formulate nanolipid carriers (NLCs)?

Currently, I am working on nano lipid carriers the issue that I am facing while formulating nano lipid carriers is that the solid lipid iam using in the formulating NLCs, is getting solidify after...

24 October 2020 1,970 19 View

RS dataset of ice cover of Arctic Antarctic?

I am looking for datasets of ice cover of Arctic Antarctic. I want only India related dataset. If you have any source of dataset please share with me. It will be very helpful for my...

13 October 2020 7,137 1 View

How can I break biotin-streptavidin bond and separate them?

I am working with a florescent compound that is biotinylated. When strepdavidin is present in the system, the binding of the protein with the compound quenches the florescence. I need to perform...

16 August 2020 6,937 8 View

Are we addressing other health dimensions adequately during COVID-19?

Too much focus on pandemic containment has sidelined other dimensions of health like reproductive/sexual health, adolescent health, mental health, geriatric health etc. Are we heading on right...

20 July 2020 2,849 3 View

Can anyone tell me if why i am getting my recombinant protein both in supernatant and pellet after lysis ?

hello all I am expressing a His6x recombinant protein and after lysis in lysis buffer i sonicate the cell suspension (40% amplitude 30 sec ON 30 sec OFF , 5 cycles). when i run SDS PAGE of the...

18 June 2020 5,494 12 View

How to use ReaxFF reactive force field in Lammps for polymer?

Hello everyone, I have build the polymer system in Material studio. My MD system consists of C. H, O, N elements. Previously , I used the PCFF forcefield and converted to DATA file readable in...

15 May 2020 1,999 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View