I am currently doing an experiment related to nanoplastics. Recently, I did an immunostaining experiment with triton X-100 to permeabilize the cells, I found out that the nanoplastics signal decreased a lot compared to without any triton x-100 under confocal microscope. Triton x-100 would destroy the membrane and the nanoplastics may be small enough to leak out through the washing step. What should I do for my immunofluorescence experiment if I wanna keep my membrane intact at the same time?
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