Why sodium hydroxide can cause increasing the fluorescence intensity for a fluorogenic reagent however acetone completely diminish fluorescence peak?

10 November 2023 1 4K Report

Why sodium hydroxide can cause increasing the fluorescence intensity for a fluorogenic reagent however acetone completely diminish fluorescence peak of the same reagent?

Adam B Shapiro

The effect of NaOH is probably due to alkaline hydrolysis of the bond linking the fluorophore to the rest of the fluorogenic reagent, generating the fluorescent product.

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Science method

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