We use the salting out technique to precipitate wide variety of protein. How does NaCl only precipitates only collagen? Does NaOH pre-treatment help to remove Non-Collagenous Proteins (NCPs) completely? Please help.
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
I am new researcher. I want to learn more and I need your help. Thank you.
04 February 2021 6,834 4 View
I am currently looking to transfect HeLa cells using lentivirus and Lipofectamine 2000 and have been looking for cited protocols. Would anyone have any that they are willing to share?
31 October 2020 5,639 2 View
Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...
11 October 2020 9,672 1 View
The antimicrobial activity testing of staphylococcus species.
02 October 2020 6,996 7 View
We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...
01 August 2020 2,087 1 View
I am interested in understanding more about NGOs that work with refugees. I am brainstorming a project that does not involve IRB approval.
31 July 2020 1,854 5 View
I got an email from someone I do not know saying my name is on her conference presentation. How do I remove it? We have the same first initial and last name (although now she has a different last...
12 January 2020 2,877 4 View
Hi all, I have a rather naive Molecular dynamics question, though functionally useful. I am simulating a rather large structure on a congested HPC, and was wondering if there is an accepted...
30 November 2019 9,877 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...
19 February 2021 9,742 2 View
I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...
15 February 2021 3,746 4 View
Hello all, I am looking for ways to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after restoration? My data set contains a...
08 February 2021 7,887 5 View
I have an alkaline solid waste which contains the following components: SiO2, Al2O3, and CaO according to the XRF analysis (as it gives the results as oxides). However, I know from the XRD that it...
28 January 2021 9,469 3 View
I've synthesized a polyurethane-Ag nanocomposite and the product is dissolved in a two-component system i.e. DMF and DMSO. I wish to know of any ways that I can use to get rid of the solvents and...
27 January 2021 1,847 3 View
gold surface is already conjugated with the PEG-2000 linker but still when I tried to conjugate it with the drug, It gets precipitated
25 January 2021 2,602 1 View
I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?
22 January 2021 152 2 View
After acid hydrolysis and incubation in the dark using Taka-diastase enzyme, 30% sulphuric acid was added to stop the enzymatic reaction. The solution was diluted to the mark and some of the...
16 January 2021 3,740 4 View
Hi ORO experts, I'm struggling with ORO background precipitates in arterial tissues. The protocol I use works very well on human atherosclerotic plaques which are full of lipids and I don't...
13 January 2021 7,110 1 View