We use the salting out technique to precipitate wide variety of protein. How does NaCl only precipitates only collagen? Does NaOH pre-treatment help to remove Non-Collagenous Proteins (NCPs) completely? Please help.
Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...
01 June 2024 7,920 3 View
My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.
27 May 2024 8,228 2 View
Need advice on user-friendly tools to ease meta-analysis process of selecting eligible and fugitive studies
02 May 2024 2,332 3 View
Hi, I am currently constructing a phylogenetic tree involving heterokonts, specifically of marine diatoms and dinoflagellates. Looking at the BLASTn results of my query sequence, I am wondering...
30 April 2024 4,239 2 View
The γ-tubulin Ab i got gives me a 48kDa band in hepa cell samples but when i try it in mouse liver tissue samples i get a weird band at 48 and another one at almost 70kDa. Ive tried a different Ab...
23 April 2024 9,475 0 View
Someone has requested a copy of an article I co-authored but I don't see options to respond. When I clicked on "View Request," it took me to a page with information about the article but not...
19 April 2024 6,368 1 View
Can the reasons for such an incident be detailed?
13 February 2024 2,795 4 View
Kindly research on how AI is going to impact on legal sevices and in particular arbitraration.Right now some firms have started using AI for research,due diligence and data analytics.
12 February 2024 4,005 5 View
Our goal is to synthesize graphene oxide (GO) utilizing biomass graphite through a modified Hummers method. However, we get rGO straight away. How is rGO produced in the absence of a reducing...
01 February 2024 7,346 3 View
There is no available sca here in the PH, and we need to isolate Actinomycetes. Thank you in advance!
20 January 2024 4,713 2 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View
Hello I have synthesized a dipeptide in an aqueous media. Now I want to precipitate and filter it. How to precipitate it. Thank you in advance. Velmurugan S
11 June 2024 525 0 View
What is the easiest way to download daily precipitation data for CMORPH, PERSIAN, TRMM and CHIRPS?
02 June 2024 4,038 2 View
I have made some compounds using DMSO , the precipitates I have got had milky skeleton. I want to know the best way to get crystallin precipitate.
01 June 2024 6,879 0 View
I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...
30 May 2024 5,169 2 View
Hello forum members, I'm conducting research on vegetable oil fingerprinting. I need annual precipitation (rainfall) data for provinces in Thailand, Malaysia, and Indonesia. Specifically, I don't...
30 May 2024 6,209 2 View
Hi. I would like to know your oppinions on freezing superstimulatory hormones (namely Pluset, Calier) and keeping them in -20°C. In was using Stimufol in our lab and it was working fine when...
30 May 2024 9,497 0 View