Can frogs transmit blood parasites to humans? If yes, how? And if not, why?
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
We are currently trying to replace acetate when dehyrdating ("drying") cryo sections on glass slides with a less harmful alternative. For many chemical purposes ethyle lactate is recommended - has...
10 June 2024 5,213 3 View
Hi everyone, I've ran a western on mouse ileal lysates and probed for COX-2. 50ug was loaded per sample and the dilution factor was 1:500 for the primary (monoclonal mouse ab) and 1:10000 for the...
13 November 2023 6,849 0 View
As for my 12Z cell line, the cells grow very well and are tenacious to the wall of the plastic culture flasks. However, they do not adhere to the wells of plates properly after seeding the cells...
01 March 2023 2,423 0 View
For the immortalized endometriotic stromal cells (22b), we have maintained them on an array of media types, such as Dulbecco's Modified Eagle Medium. Nutrient Mixture F-12 with 10% fetal calf...
01 March 2023 4,805 2 View
Hi, I am doing a freelance project on bone defects treated with control and treated bone sponge- so I know little about the actual study and bone is not my forte in science. I'm told the...
10 February 2023 2,892 1 View
I am recently new in trying my hand at fiber photometry, but recently implanted fiber optics as well as viruses to monitor both dopamine and serotonin neurotransmission. I successfully implanted...
11 December 2022 8,954 1 View
I have some buccal swab samples that are in a liquid preservative. Our group is trying to extract the DNA from these samples using the E.Z.N.A Tissue DNA kit and Forensic DNA kit. Both protocols...
26 January 2022 9,843 1 View
Hello all! I am struggling to understand why my bis-tris gel is not resolving well. There is not a uniform band forming in the lanes causing the gel to turn completely blue. I am using this...
20 December 2021 2,889 1 View
Hello all! So I am looking at very small proteins (~15kD) and just switched to the Bis-Tris system. I poured my own 12% gel, made up MOPS running buffer, and went ahead and bought the NuPage...
08 November 2021 4,125 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,519 3 View
A group of students prefer frogs for dissection.
16 July 2024 4,848 1 View
I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...
07 July 2024 5,243 0 View