Can "dirty" activated carbon (GAC) add contamination to water as it is being treated?

We have a system to treat water from an old industrial basin before discharging to the river. The system initially consisted of (in this order): a 3-stage bag filter, 3 granular activated carbon...

22 July 2019 9,298 3 View

Is it possible to mark shared first authors of a published article?

Is it possible to mark shared first authors or joint senior authors of published articles on your research profile?

04 April 2019 8,967 6 View

MYCOLOGISTS/BIOLOGISTS NEEDED: To help identify the fungal species pictured in the 5 following photographs?

My family and I would like to welcome any and all responses, advice, and any additional information that you would like to share. We would also like to thank you from the bottom of our hearts (in...

19 March 2019 2,640 5 View

How do I correct RNA pooling in wells during RNA Gel Electrophoresis?

I am running an agarose gel to check for RNA degradation, and I'm having trouble getting my sample to migrate out of the well. I've attached a copy of the picture showing the sample in the well....

19 February 2019 4,876 4 View

Method to determine low cellulose concentrations (0.5-1.5%) in feed?

I'm looking for a method that is sensitive enough to measure dietary cellulose content of rat feed at 0.5% cellulose, and up to 1.5%. We have tried the ADF method and AOAC 991.43, the ADF was...

21 May 2018 2,237 1 View

Malaysian version of the Rosenberg Self-Esteem Scale?

Dear Colleagues, I am looking for the Malay version of the RSES, preferably the one Prof. Viren Swami (2011) studied in his chapter: Further Examination of the Psychometric Properties of a Malay...

06 November 2017 6,494 2 View

I am looking for a method to stain spleen sections to identify germinal centers. Which stains and/or antibodies would you recommend?

26 September 2016 3,022 3 View

I have problems culturing rabbit hybridomas. Do they usually present slow growth?

Does anyone have specific techniques or media to help the cell line increase its growth?

31 August 2016 8,770 1 View

Does anyone know where I can get MDA-MB-231 LUC cells that are able to develop tumors in the subcutaneously in the flank of nude mice?

09 June 2016 9,437 2 View

Does anyone have information about the PCL-5 and the Centrality of Event Scale in Arabic?

15 October 2015 5,571 18 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View