What selectable marker genes do you suggest instead of hygromycin in the gene knockout project in rice?

15 May 2024 0 3K Report

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Science method

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Abnormal changes in texture and color of chicken meat?

Dear Sir and Ma'am, We faced a problem in the industry that couldn't be solved despite running several tests. "When using chicken thighs for making sausages, we noticed weak tissue in certain...

11 January 2024 4,415 0 View

After designing gRNA, how should we make it?

After designing gRNA, how should we make it? I need an article, site or instruction on this please

26 September 2023 9,258 0 View

What are the ways to confirm the edited gene through CRISPR system?

Please suggest me some articles or sites about this topic.

22 September 2023 2,553 1 View

What are the ways to confirm the edited gene through CRISPR system?

22 September 2023 3,290 3 View

What is knowledge gap in tourism research?

what is the knowledge gap in tourism research?

10 May 2023 4,024 4 View

How can I find proteins in Artemisia annua plant that are involved in primary or secondary metabolites?

I have to draw a phylogeny tree using their complete sequence on the NCBI website

19 April 2023 8,745 0 View

How to get consistent result from CD spectroscopy?

I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...

07 December 2021 9,234 7 View

How to differentiate the emission peak of a compound from the emission peak of the light source(instrument)?

I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...

21 November 2021 9,368 3 View

Hi, I am looking for this book "introduction to magnetic material" by Cullity?

Hi, I am looking for this book "introduction to magnetic material" by Cullity , how can I find it?

16 January 2020 229 2 View

Hi, I am looking for a solution manual of this book "introduction to magnetic material" by Cullity , how can I find it?

16 January 2020 3,695 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View