Why do they immunize mice by intraperitoneal injection?

Under what conditions will we use the method of intraperitoneal injection of vaccination in mice? In streptococcus suis research, some studies vaccinated mice by intrapetitoneal injection? why?

24 February 2018 8,500 6 View

A protocol for NKT cell depletion?

I am now planning to deplete the NKT cells in c57 mice and then injected with tumor cells. I searched online, and found some people used NK1.1, some used CD1d. I am now confusing as NK1.1 antibody...

20 December 2017 8,803 1 View

What is the maximum volume of intramuscular injection in mice?

What is the maximum volume of intramuscular injection in mice? dose translation from pig to mice?

06 September 2017 6,759 2 View

Does promoter-cre expression affect the the original gene expression?

I am confused by the Cre-flox system. For the conditional knockout (Cre-flox) system, Cre is expressed under a specific promoter. It is knocked-in using the homologue recombination, so the...

25 April 2017 9,244 3 View

What method can estimate bicycle traffic volume?

There are so many methods in estimating car traffic volume.However, methods about estimating bicycle traffic flow are very few.So I want to know some methods can estimate bicycle traffic volume.

16 March 2017 2,546 8 View

How to prepare 5ng/ml PMA from Sigma P8139?

Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...

21 February 2017 584 2 View

Which primer should I use if I want to detect the mRNA level of Akt in HCC cell lines?

Dear All, In my western blotting experiment I found a decrease level of both Akt and pAkt expression. So I want to check the mRNA level of them. I am wondering which primer should I buy for the...

16 October 2016 3,622 4 View

What is the minimum amount of starting material to use in flow-cytometry?

16 October 2016 2,440 3 View

What expression proteomics techniques to use in characterizing the protein profile of iPSC-derived leukemic cells?

06 October 2016 167 1 View

Nafion can't be used in high temperature in ORR testing, how can I solve it?

I want to coat some Pt catalyst on the glassy carbon electrode, and test it at high temperature (>140 degree). Usually, nafion is used to fix the catalyst on the GC. But as I know, nafion can't...

16 July 2016 2,190 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View