Dear all,
I wonder if it is convenient to use BL21(DE3)PlysS without adding glucose to express protein of interest? if its okay, do you have a reference ?
How do companies or scientists predetermine DNA ladders for Gel Electrophoresis? It makes no sense how they would know? I mean DNA is so incredibly small. As it separates in the gel how can they...
30 May 2024 1,317 0 View
I want questionnaire for quatitative research
14 February 2024 505 1 View
Rubber wood is main raw material for production. As rubber wood is prompt to Fungi attack, is there any risk of using different wood age (eg 1 month vs 3 month) after receiving the load for...
07 February 2024 3,390 0 View
I modelled a 3d truss with one storey concrete structure attached to it as shown on the model. I know that the one storey concrete structure changes the rigidity and the flow of the forces on the...
02 January 2024 6,810 1 View
Hi I am trying to work out what my effect size was for my study I have the sample size, power etc... its there a program that can work out what it is?
04 December 2023 9,249 3 View
We were making up the buffers for our Promega PureYield plasmid midi-prepkit and accidentally added 315ml of isopranolol to the endotoxin wash buffer instead of 210ml. Do you think we can use it?
04 December 2023 9,317 1 View
How does the nitrogen in pig manure effect the soil
27 November 2023 1,084 8 View
Hi everyone, In my readings about C. diff. ribotypes, I found a ribotype the researcher refers to it as RT 014/020. I wonder if it refer to 014 and 020 strains or 014 to 020 strains or what...
12 November 2023 3,253 0 View
What are the methods for measuring the swelling of carbomer powders? can result methods, articles.
11 July 2023 7,831 2 View
Hello everybody, I purified my protein and when I run it on SDS-PAGE, a small molecular weight sized band (10 kDa) appears. Do you have any idea what that could be? an image of the SDS-PAGE is...
09 July 2023 5,325 3 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
how i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water
08 July 2024 9,104 2 View
I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...
26 June 2024 3,664 1 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View
I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...
12 June 2024 7,331 1 View
Hi all, I have been attempting to do field EPSP but I am not able to elicit a response. I am using a thin walled electrode for the recording electrode at 1.5mOhm resistance. I have filled the...
10 June 2024 2,418 0 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View
Hi, we're trying to determine two amiglucosides antibiotics and one aminoacid in a powder mix whose principal ingredient is glucose: 60 % w/w. The antibiotics are amikazine and apramycin, the...
22 May 2024 5,636 0 View
After lapse of a few months, we recently attempted insulin-tolerance tests in C57BL/6N female mice at 6 months of age. We began, years ago, with Humulin-r at 0.75 U/kg. In our recent tests we...
22 May 2024 3,797 3 View