Dear all,
I wonder if it is convenient to use BL21(DE3)PlysS without adding glucose to express protein of interest? if its okay, do you have a reference ?
Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !
01 March 2021 2,501 3 View
Currently using Msc Marc Mentat for FEA. Now i conduct the mesh convergence/sensitivity analysis manually. So I need to know if there any automatic way to conduct the mesh convergence/sensitivity...
04 February 2021 3,310 3 View
My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!
04 September 2020 4,199 4 View
If i use the MHD module, i will have to provide the values of external magnetic field, but isn't the magnetic field established as a consequence of the current flowing through the air? How am i...
15 August 2020 200 1 View
Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...
03 March 2020 2,329 3 View
Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...
18 February 2020 8,725 2 View
I am one of the authors of the following article whose name is not recognized on the system. Although the article has been uploaded, the system has not recognized my name. Please review and add...
03 January 2020 8,737 4 View
I'm troubleshooting immunofluorescence staining for some antibodies. Some work fine with my protocol [H2AX, Ki67, 3-nitrotyrosine], some stained once and never again [8oxoG], and some won't stain...
21 April 2019 5,510 3 View
From Bio-Rad's user guide (see below) it is not clear to me what is the best activation time to use. It states that 1 min gives optimal results for WB followed by immunodetection, but that 5 mins...
25 March 2019 1,508 4 View
I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...
02 March 2019 5,069 1 View
Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...
16 February 2021 453 2 View
Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...
07 February 2021 9,989 7 View
Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...
30 January 2021 9,535 2 View
Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...
27 January 2021 2,525 4 View
Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...
25 January 2021 7,608 3 View
Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...
06 January 2021 6,511 4 View
I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...
23 December 2020 8,440 4 View
I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...
21 December 2020 4,647 2 View
Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?
08 December 2020 6,312 3 View
I have been working with the C2C12 myotubes and measured their glucose uptake activity using an indirect glucose uptake assay (measuring how much glucose is left in the medium after stimulating...
23 November 2020 2,118 3 View