PDA Detector: Lack on a Wavelength Range, why?

Hello, We have a Shimadzu UHPLC equipment. Recently, the PDA detector lamps have been changed and all tests recommended in the manual have been performed. Apparently that's fine. In the first...

09 September 2019 1,836 3 View

What may have cause the big black blob at the bottom of the lanes wherein no PCR product is detected?

This is a agarose gel electrophoretogram of my PCR products using an SSR marker. This is just for checking if amplification is successful for all the DNA samples examined. This will still proceed...

23 June 2019 8,833 2 View

Stve Wolinetz wrote a review of my book with Fred Engelmann, he is not a coauthor. Why do you mix together authors and reviewers?

You should treat reviews as distinct from what an author is reviewing.

09 June 2019 9,191 5 View

How can shearing of DNA during extraction can be prevented?

I am currently working on dna extraction from soybean leaves. Months prior, I could get clean and clear bands of DNA extracts when viewed in the agarose gel. But this time around, when I checked...

13 November 2018 3,771 8 View

Would alcohol administration affect GAPDH expression at the protein level?

Loading control for Western Blot

22 October 2017 7,399 3 View

Viral infection giving low protein content after lysis for western blot?

22 June 2016 9,736 4 View

In Profile curvature a concave surface accelerates or decelerates the flow?

Hi, this site from ESRI explains the curvature, my question is about the profile curvature. They say that positive values represent a concave cell, were the flow will be accelerated. Negative...

19 April 2016 3,208 3 View

Can variances/ SDs be treated as interval scaled?

Hi, I am analyzing temporal trends in parameters like the autocorrelation and the variance. I know that, albeit they are calculated for interval scaled variables, correlations are not interval...

11 April 2016 7,683 4 View

Recommendations homogenizing muscle for zymography?

We are using zymography to look at proteases in skeletal muscle samples. Unfortunately, while the positive controls are working, the samples with muscle extract are not. There are a couple of...

23 October 2014 8,138 6 View

Is anyone interested in taking part in a field study in project management?

I am a PhD candidate. I am writing a dissertation: "What are the factors of failure in the implementation of new project management technology utilizing dashboards as a tool to measure key...

07 August 2013 1,480 2 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How to do immunofluorescence staining of the microtome sections of paraffin-embedded tissue ?

I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...

10 February 2021 3,113 3 View

How to record RBC autoflourescence in a tissue sample?

RBCs have auto-fluorescence, I want to detect that auto-fluorescence in paraffin embedded tissue samples. Any suggestions?

08 February 2021 9,357 1 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How do I keep my prefixed (PFA) frozen tissue sections from lifting off the slide?

I'm trying to stain mouse lung tissue IHC-IF I fix in 4% PFA overnight immediately upon harvesting tissues Then dehydrate/cryoprotect in sucrose gradients 15% to 30% Then freeze in OCT media...

06 February 2021 2,336 3 View

How to use the Embedded Region constrain on Abaqus CAE?

Hello fellow academics, For my Final Year Project, I am looking into different ways of modelling the interface between PVA and gelatin. I have successfully modeled the interface using the "tied"...

04 February 2021 8,698 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View