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Questions related to Bioinformatics
Hallo, for NGS validation I need public refence genomic data (annotated!), especially the variants annoted against GRCh 37. I am interested in the data from the HUREF and CEPH/UTAH pedigree 1463....
03 March 2019 4,568 2 View
Hello everyone, I was wondering what kinds of information can be deduced from a gene's sequence? I am studying Biology and was assigned a Bioinformatics task for my Molecular Biology project...
03 March 2019 3,984 3 View
Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...
03 March 2019 4,955 5 View
As we know from the Bruker's instruction or CLSI, the recommended bacterial load for identification by MALDI-TOF MS is around 10^5 CFU. Moreover, it is also clear that species identification would...
03 March 2019 7,838 2 View
Can anyone here help me to do LEfSe analysis on my 16S rRna data?I am ready for a collaboration
03 March 2019 8,790 5 View
Suppose 2 genes produce 2 proteins which would be binding. After the genes are turned on and the proteins are produced, then genes can be turned off and the proteins produced can bind with each...
04 February 2019 348 7 View
I need to know the number of gene copies that encode an S-layer protein in certain bacteria whose genome is already annotated in NCBI Also, how can I know whether some of them are truncated or...
02 February 2019 6,637 5 View
Scope of the course should include Human Exome&Genome analyses with basic computational biology&big biological data anlyses. It also should focus on human and clinical outcomes....
02 February 2019 3,479 5 View
Hi, Could anybody please help with the basic understanding how to process AP-MS (affinity purification mass-spectrometry, e.g. mass-spec done on the pull down with an antibody of interest) data. I...
02 February 2019 4,280 4 View
Kindly help me to design a bioinformatics methodology for the list of frame shift mutation associated with a specific disease. These variant are found in a protein which has disorder regions. Can...
02 February 2019 3,088 3 View
Hello colleagues, I feel like it's a stupid question but I couldn't google the answer quickly. Are there any conventional rules about multiple sequence alignment visual presentation - sequences'...
02 February 2019 4,355 1 View
Hello, I used to molview.org for drawing the structure of molecule and saved it as ".mol" format. Than I convert to pdb with PyMoL. But I'm not sure if this is valid? Is there an alternative...
02 February 2019 8,532 1 View
02 February 2019 8,926 6 View
Hello! I have bacterial (16s V3-V4) and fungal (ITS2) miseq data from soils collected on different farms where I'm hoping to compare microbial networks in different farming types (conventional and...
24 January 2019 6,911 6 View
Hi, I have a Transcription factor binding site identificated. And I got a track from Drosophila genome with all the locations of this binding site. I would like to compare the acumulation of my...
21 January 2019 1,688 3 View
Currently, I am testing Pst DC3000 cfu assay on Arabidopsis Col-0. DC3000 was grown on KB (Rif) media, and I infected with OD600=0.001 density by hand syringae infiltration. 0 day cfu result...
03 January 2019 3,402 3 View
plz help
01 January 2019 7,469 0 View
Dear all, I’m performing a cancer genomic analysis and I need a negative control of non-cancer genes (200-400 genes). So, I appreciate any help. S.
01 January 2019 8,846 5 View
Can anyone suggest me a tool to check the nature of promoter (Inducible or constitutive) through bioinformatic or computer tool.
01 January 2019 8,937 3 View
Greetings! I am performing an homology modelling on a protein with unknown structure. A large part of this protein has already been crystallised. My sample has 80% query coverage with the...
01 January 2019 5,201 4 View
I want to pursue my career towards something related to computer science and biomedical combined. I looked at options and bioinformatics interests me, but i do not know much about it. I have...
01 January 2019 7,738 4 View
I have a dataset of immunoprecipitation assay from LC-MS/MS. I would highly appreciate any advices regarding all possible computational analyses (including the best tools and resources) that I can...
01 January 2019 975 2 View
I am an HPC user looking to migrate from a university-supported UNIX system to a cloud-based system. What options are the most reliable and affordable? Thank you.
01 January 2019 6,926 8 View
Hi everyone, I'm a new research intern who's been tasked with analyzing some RNA-seq data. I'm running DESeq2 analysis through an automated pipeline, however I keep returning massive fold changes...
01 January 2019 8,432 3 View