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Questions related to Bioinformatics
I want to identify gene duplication events of specific genes between particular plant species. I also want to calculate the Ka and Ks value between the duplicated genes. It would be a great help...
03 October 2021 6,906 3 View
Transition metals share close properties although have different response during photocatalytic degradation, which of them are best for photocatalytic degradation of aqueous organic pollutants?
30 September 2021 9,659 3 View
Currently I have the code to compare the ED50 values among different treatments = compParm(res2, strVal="e"). What would the code be for a different ED value?
29 July 2021 9,691 1 View
I am getting positive binding energy in Autodock. Is it alright?
08 July 2021 7,647 4 View
Dear experts, I am trying to set up my annealing protocol to make dsDNA out of 2 ssDNA oligos (29 nucleotides each). I have both oligos in the same 100 uM concentrations and use 40 ul of each....
07 July 2021 5,684 1 View
Good morning to everybody, I am working on article with tobacco and Arabidopsis genomic data and. Does anybody know database, server, or programm where I could find orthologs of Arabidopsis...
05 July 2021 6,951 2 View
I have installed the tool Raccoon in ubuntu. I was able to open it and load ligands, receptors , grid and dpf files successfully. However, I could not able the run the command ./RunVS.sh as it...
27 June 2021 735 0 View
I have a 600Mb genome assembly and I'm trying to annotate it. I used mercator, Pokka and RastK, but it's either not compatible or the sequences are larges.
18 June 2021 1,367 4 View
Our power supply we were using to do overnight transfers is broken, and unfortunately was discontinued. For western blots, we transfer overnight in the cold room at 4C with a constant current of...
03 June 2021 7,267 1 View
For PTM site prediction through In Silico method, I try to use this server but it is not responding. Please help me to solve the problem. Thanks in advance.
23 May 2021 2,802 4 View
In western blot, primary antibody usually incubates overnight at 4°C. But the signal after incubation for 1-2 hours at room temperature is also strong enough to detect. If I incubate at room...
13 May 2021 1,680 3 View
one of my student wants to do Pulso-Typing of clinical isolates of S.typhi. i would like to know where this facility is available in Pakistan.
20 April 2021 5,374 0 View
Hello. Everyone. I'm struggling to find some effective methods for detecting mutations in a plant genome or gene level, using mutated plant lines, without WGS or NGS. Is there any recommended...
29 March 2021 2,895 10 View
hi every one I choose a virus to do bioinformatic for it. it has a lot of protein. how should i choose one of them to do docking and MD for? how should i understand which protein is better than...
13 March 2021 5,076 6 View
Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...
28 February 2021 2,336 1 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
23 February 2021 4,733 3 View
Hi everyone, I am working with data obtained from the Geo (NCBI). This data is gene expression found with the Affymetrix Human Genome U133A Array and is analysed to get a log2 from the raw data...
23 February 2021 9,143 3 View
Copy number variations (CNVs) are the gains or losses of genomic regions which range from 500 bases upwards in size. Whole-genome studies have revealed the presence of large numbers of CNV regions...
21 February 2021 7,421 2 View
Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...
09 February 2021 2,938 5 View
I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...
29 January 2021 8,007 2 View
bioinformatics has a big importance in human science and lots are seeking to study that field, so it attracts me to know it's relation with poultry science and how we can benefit from it in...
21 January 2021 7,183 3 View
As i am working on protein-ligand docking, i want to input the x,y,z coordinates in autodock and to do that, i need the coordinates of aminoacids present in the binding site. So, PLease help me out.
04 January 2021 4,092 4 View
I want to know about the Software used for drawing plants or plant part, and presenting graphical figure of data. Thank You
27 December 2020 7,709 6 View
I have multiple AMPs peptides. I want to know the origin of each one; any bioinformatics web tool helps to do this.
12 December 2020 6,109 1 View