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Questions related to Bioinformatics
Deleting the residue using pymol leaves the loop broken and I don't want to insert another residue but connect the loose ends. Purpose: to reduce loop shaking. Would anyone have a suggestion?
10 July 2023 9,753 2 View
Capsicum substitute
09 July 2023 4,155 4 View
DNA was extracted from soil using DNeasy powersoil pro kits. The ladder used here is the GeneRuler 1kb plus. The DNA smear starts at the 10'000bp marker and extends down to the 2000bp marker or...
05 July 2023 9,075 4 View
Hello; I have built different regression models Linear regression, MLTP, and Stack regressor. I need to compare the matrics and performance of these models. How I can do that scientifically and...
01 July 2023 2,901 5 View
I am having this problem since the soil samples that we have collected contain a lot of roots and other plant debris which might affect our data. Our study focuses only on soil eDNA.
29 June 2023 8,407 1 View
I run repeated measure ANOVA with between subjects (gender) as independent variable to test its effect on the willingness to pay (WTP) for two conditions A and B (within-subjects) where the same...
24 June 2023 3,350 4 View
I made detection primers to detect Antimicrobial Resistance Genes in soil that I used in conventional PCR. But, my primers have different results in different soil samples (I extract DNA in the...
09 June 2023 1,737 2 View
CPORT is algorithm used for to predict the active-passive residues of protein. But now the server is under maintenance. Is there any other server apart from CPORT to predict active and inactive...
14 May 2023 8,195 0 View
1. I have used anti bacterial & anti mycotic [ pencillin streptomycin & amphotericin B ] at required concentration still I face contaminations , so is there any other way out to prevent...
06 May 2023 7,577 1 View
I have been running gel zymography (7.5% polyacrylamide co-polymerized with 1% gelatin) loaded with 250 µg of protein / well from colonic lysate with the hopes of visualizing MMP-2 and -9. I had a...
21 April 2023 8,314 5 View
I mean Andrew Fisher's formulae for sample size determination
15 April 2023 7,458 2 View
Plz suggest me..
11 April 2023 3,440 5 View
Hello everyone I have been working with the Caco-2 strain for a few months, but unfortunately, the cells were already contaminated. I already tried to decontaminate with penicillin/streptomycin...
05 April 2023 934 1 View
We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier,...
28 March 2023 2,479 1 View
Hello. I am docking two proteins and then comparing the results with another docking study where one of the proteins has an SNP. For the first protein (which is then mutated), there is a motif...
27 March 2023 6,685 0 View
What to do if ChimeraX software doesn't recognise the .chimerax file downloaded from SwissDock after docking? Besides, the zip file of prediction done was empty. Thank you.
19 March 2023 5,286 3 View
Can anyone please give me a software or website which converts Ref sequence protein ID to uniprot ID. For example ref seq protein ID Uniprot ID NP_853513 Q7Z3Y7 I...
11 March 2023 2,951 1 View
Especially isolation source, host, and environment etc,... with emphasis on Rhizobia if applicable.
10 March 2023 6,356 1 View
Can anyone please suggest me methods to remove excess proteins from protein-bound liposome. 100 nm liposome (POPC+1 mol% biotinylated lipid) contains biotinylated lipids. The surface of the...
03 March 2023 7,208 1 View
I am trying to calculate protein-ligand contacts (especially hydrophobic interactions) in the allosteric site of a GPCR. Thus, could you suggest a tool that I can use free of charge, except...
27 February 2023 8,230 4 View
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20 February 2023 7,690 3 View
Hi I was wondering if you could recommend me a standalone seq converter that allows me to convert the mentioned file formats, I rather use a command line tool since I'm converting multiple files....
17 February 2023 6,006 0 View
Attached here is the kit I've been using
08 February 2023 3,210 5 View
Hello there. I was looking for a simple way to get all chromosome 21's genes with their size and their protein size (amino acid number). also It's all functional and non-functional mRNAs. Is...
05 February 2023 8,866 6 View