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Questions related to Bioinformatics
I run repeated measure ANOVA with between subjects (gender) as independent variable to test its effect on the willingness to pay (WTP) for two conditions A and B (within-subjects) where the same...
24 June 2023 3,266 4 View
I made detection primers to detect Antimicrobial Resistance Genes in soil that I used in conventional PCR. But, my primers have different results in different soil samples (I extract DNA in the...
09 June 2023 1,629 2 View
CPORT is algorithm used for to predict the active-passive residues of protein. But now the server is under maintenance. Is there any other server apart from CPORT to predict active and inactive...
14 May 2023 8,132 0 View
1. I have used anti bacterial & anti mycotic [ pencillin streptomycin & amphotericin B ] at required concentration still I face contaminations , so is there any other way out to prevent...
06 May 2023 7,521 1 View
I have been running gel zymography (7.5% polyacrylamide co-polymerized with 1% gelatin) loaded with 250 µg of protein / well from colonic lysate with the hopes of visualizing MMP-2 and -9. I had a...
21 April 2023 8,207 5 View
I mean Andrew Fisher's formulae for sample size determination
15 April 2023 7,378 2 View
Plz suggest me..
11 April 2023 3,378 5 View
Hello everyone I have been working with the Caco-2 strain for a few months, but unfortunately, the cells were already contaminated. I already tried to decontaminate with penicillin/streptomycin...
05 April 2023 836 1 View
We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier,...
28 March 2023 2,406 1 View
Hello. I am docking two proteins and then comparing the results with another docking study where one of the proteins has an SNP. For the first protein (which is then mutated), there is a motif...
27 March 2023 6,606 0 View
What to do if ChimeraX software doesn't recognise the .chimerax file downloaded from SwissDock after docking? Besides, the zip file of prediction done was empty. Thank you.
19 March 2023 5,038 3 View
Can anyone please give me a software or website which converts Ref sequence protein ID to uniprot ID. For example ref seq protein ID Uniprot ID NP_853513 Q7Z3Y7 I...
11 March 2023 2,857 1 View
Especially isolation source, host, and environment etc,... with emphasis on Rhizobia if applicable.
10 March 2023 6,250 1 View
Can anyone please suggest me methods to remove excess proteins from protein-bound liposome. 100 nm liposome (POPC+1 mol% biotinylated lipid) contains biotinylated lipids. The surface of the...
03 March 2023 7,137 1 View
I am trying to calculate protein-ligand contacts (especially hydrophobic interactions) in the allosteric site of a GPCR. Thus, could you suggest a tool that I can use free of charge, except...
27 February 2023 8,153 4 View
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20 February 2023 7,593 3 View
Hi I was wondering if you could recommend me a standalone seq converter that allows me to convert the mentioned file formats, I rather use a command line tool since I'm converting multiple files....
17 February 2023 5,940 0 View
Attached here is the kit I've been using
08 February 2023 3,135 5 View
Hello there. I was looking for a simple way to get all chromosome 21's genes with their size and their protein size (amino acid number). also It's all functional and non-functional mRNAs. Is...
05 February 2023 8,802 6 View
I know that in LighterCycler 480 system, we can use Absolute quantification/fixed points methods and then set a reasonable noise band to estimate the CT values. My workmate told me to use this...
02 February 2023 2,532 3 View
I am working on immobilization of peroxidase on magnetic nanoparticles using adsorption. Unfortunately, the change in enzyme activity is very low. Consider the absorbance change over 3 mins for...
02 February 2023 1,071 3 View
Using AlphaFold, I have predicted protein structure models and lDDT plot. Is there a way to color each model based on lDDT values using PyMOL? Thank you in advance.
27 January 2023 8,701 4 View
Hello, collegues, I'm trying to estimate the distribution of a certain kind of microorganism (eg Methanobacterium) in a certain environment (permafrost) from metagenomic data presented on various...
19 January 2023 3,528 2 View
I'd like to sequence the genome of the gopher tortoise. The genomes of congeners are ~2.4Gb. I'm trying to decide how much coverage is necessary; we plan to run the sample on a portion of a...
13 January 2023 636 4 View