Which machine is more better for real time PCR..?

i am going to buy a real time PCR machine for diagnostic purpose, so please suggest me which is advanced, better and more powerful for diagnostic purpose.

10 November 2018 8,100 5 View

Anybody can suggest a good Giemsa trypsin banding protocol ?

I am using SSC buffer to make a giemsa (20mg per 50 ml SSC buffer). we use aged slide (65 degree for overnight) for treating with Trypsin Solution for 20 sec at 20 degree. We get minor banding...

07 August 2018 4,400 4 View

PGMY11 primer set doesn't give any band with DNA sample which are shown positive with GP5+ and GP6+ primer set of cervical brush sample???

i screened many DNA samples extracted from the cervical brush sample for presence or absence of HPV DNA with primer set GP5+ /6+ and i got five positive sample. then i used that 5 positive samples...

07 August 2017 6,354 2 View

Any one have idea regarding sequence of Primers for Tuberculosis PCR?

There are many primers available in paper published, but most of all have problems with Tm, product size, nonspecific binding and many more. I need a best primer sequences for TB PCR. Is there...

02 March 2017 254 2 View

RAPD analysis of binomial matrix and dendogram preparation?

I have two variety, one is A - in vivo plant leaf and B - in vitro plant leaf. i had extracted DNA from these both samples A & B. Done PCR with 31 RAPD primers and got varies band pattern on...

08 September 2016 7,948 6 View

16s r-DNA PCR product giving three bands on gel, so which one should be considered?

I have Extracted DNA from six different actinomycetes bacterial strains and then do PCR with 27F & 1492R primers. i should get the band with size between 1400 to 1500 bp. but here i got three...

07 August 2016 9,941 6 View

RAPD analysis for the in-vitro and in-vivo plant samples of the same plant species?

I have done RAPD Analysis for one plant with its Invivo tissue and in vitro tissue samples. I have used 31 primer sets, so now i dont getting how much maximum variation can be considered for this...

07 August 2016 825 5 View

What should be the concentration and content of all PCR components for doing RAPD analysis?

i am going to do RAPD analysis of two different plant species with 31 different RAPD primers. I need to know the best suitable concentration and volume of primer, MgCl2, Taq pol, PCR buffer, and...

05 June 2016 914 3 View

Which HPLC method, column type and solvent system will best suite for Alginate analysis extracted from different bacterial strains?

05 June 2016 9,627 3 View

How can I remove a high polysaccharide content from DNA extracted with plant leaf?

I have extracted plant DNA from leaf sample. i got good precipitates when precipitated with ethanol and iso propanol. but when i dissolve the dried pellet in TE or molecular grade water, it became...

11 December 2015 4,297 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View