How many PBMs to inoculate for 5 days in a 96-well plate in 200 µl of medium?

14 August 2023 1 7K Report

How many PBMCs can I seeded for 5 days in a 96-well plate in 200 µl RPMI with FBS and PHA?I started with 30,000, and I don't know if it's not enough

A.B Bayazid

Dear Dr. Aneta Rogalska

Since it seems you can't change the media I would suggest you use 6well plate.

Kind regards

AB Bayazid

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