Sodium citrate buffer absorption coefficient value?

22 August 2023 1 6K Report

Hi all! I recently started performing enzymatic treatment on Nannochloropsis oceanica in order to disrupt the cell wall. Throughout the different extracellular components I want to analyze, there are also pigments, more specifically chlorophylls (a and b) and carotenoids+xanthophylls. Since I'm using sodium citrate buffer as treatment medium (0.1 M, pH 5.5), I need the correct absorption coefficient values in order to calculate the concentrations of the different pigments.

The problem is that I'm having trouble in finding that nfo online and I was hoping somebody could help me.

Kc Chico

The formula is here if you need to calculate it: https://www.aatbio.com/resources/faq-frequently-asked-questions/What-is-the-absorption-coefficient

Or were you looking for a table somewhere?

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