Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

What is the best lighting design for conservation?

In the museums, the sensitive organic materials need to be displayed under 50 lux or less and aways from any UV or IR. LEDs still have a little amount of UV. What is the better option? Is it...

20 January 2021 5,965 3 View

Cryopreservation of cancer cell lines @-80?

07 January 2021 5,410 8 View

Can a researcher depends the conventional PCR as molecular based technique for quantification of STAT3in stead of real time PCR in FFPE tissue ?

The FFPE tissues are relatively difficult to extract the RNA from so, how one can use reverse transcription based PCR

10 December 2020 4,398 6 View

Can anyone help me to solve this problem?

when i did time history anlysis by using sap 2000 , the program showed the first two step only at .02 and .04 . can anyone help me to solve this problem?

27 November 2020 3,810 2 View

Analytical solution of stresses distribution due to tunneling in expansive soil?

I want to know how can I do an analytical solution for stresses distribution due to tunnel excavation in an expansive soil to compare with a numerical simulation. and which method I can use?

22 September 2020 7,602 2 View

Meshing problem in abaqus?

Can any one help me in meshing in ABAQUS, please?

09 September 2020 7,957 7 View

Why halogen species lower the flame temperature?

Chlorine, a component of PVC polymers, acts like a halogen-based flame retardant agent in the event of fire.

07 September 2020 5,450 6 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

How do I determine Gestational Day 0 in Rats and Mice to start treatment?

Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...

15 October 2020 7,402 4 View

How many matings does it take to get a successful pregnancy between 129 and Cast/EiJ mice?

I'm breeding female 129SvJae and male Cast/EiJ mice to get an F1 cross, and we've had many plugs formed but no successful pregnancies. They are being bred in an animal facility with high success...

12 August 2020 7,156 1 View

How can chemical laboratory experiments be done on pregnant rats?

How can chemical laboratory experiments be done on pregnant rats? Upon knowing the effect of a chemical substance, protein, hormonal or enzyme on animals, experiments with pregnant rats .. Do any...

22 July 2020 6,030 1 View

What are the mechanism that prevent immune response against commensal microbesand fetuses?

What are the mechanisms that prevent immune responses against commensal microbes and fetuses?

21 June 2020 7,167 3 View

Is the range of pregnancy week and sample size cited in this article?

15 February 2020 1,301 1 View

Regarding pregnancy-induced changes that affect drug disposition, are there disease conditions that result in similar physiological changes?

I am wondering if there are chronic disease conditions that exhibit pregnancy-induced physiological changes known to influence drug disposition during pregnancy. If such disease conditions, can...

10 November 2019 2,928 1 View

Pregnancy strip test engineering?

Hi, please does anyone has a detailed idea about how pregnancy strip test is made? I mean, how they calculated the exact amount of hCG antibody to be embedded in the pad? Thanks

31 December 2018 1,396 1 View

Hi friends I have a question,Is there a difference between neurons and astrocytes of mouse and rat's?

Why is it better to isolate neurons from rats on the 18th and not 21days of pregnancy?

11 December 2018 9,614 0 View

Hi, will you be asking them about pregnancy sickness as part of the information gathering?

If so, will you use any instruments to assess the severity thereof? It would be interesting to see if hyperemesis gravidarum is associated with placental function. In 12,000 women you would expect...

09 October 2018 6,990 5 View