Now I have sodium hyaluronate powder which is 75kda to kda, what solvent should I choose to make a 1% sodium hyaluronate solution. Is PBS solution or water suitable in this case?
Sodium hyaluronate is a water-soluble compound, so you can prepare a 1% sodium hyaluronate solution using either water or a phosphate-buffered saline (PBS) solution. Both options are suitable, but the choice between them depends on the specific requirements of your experiment or application:
Water (Distilled or Deionized): Using water to prepare your 1% sodium hyaluronate solution is a simple and common approach. It's suitable for many applications and is often used in laboratory settings. Just make sure the water is of high quality, such as distilled or deionized water, to avoid introducing impurities.
Phosphate-Buffered Saline (PBS): PBS is a buffer solution commonly used in biological and biochemical applications. It contains salts and a phosphate buffer, which can help maintain a stable pH and provide an environment that mimics physiological conditions. If your application involves biological samples or you want to maintain a specific pH, using a 1% sodium hyaluronate solution in PBS can be a good choice.
Ultimately, the choice between water and PBS depends on the requirements of your experiment or application. If you're uncertain, you may want to consult with colleagues or experts in your field to determine which solvent would be most appropriate for your specific research or project.