i am working on the determination of the synergism activity of the herbal and allopathic drug. firstly i tried to find out the zone of inhibition of individual drug having lower (25, 50, 100 ppm), middle (250, 500, 1000 ppm) and upper (2500, 5000, 10,000 ppm) concentration against s. aureus bacteria. but the expected result was not obtained in case of herbal drug. kindly provide the valuable suggestions for the same with references. I tried the same experiment against E. Coli and Pseudomonas Aeruginosa & the expected result was obtained in both cases. I am facing a problem only in case of S. Aureus. I also like to add that i repeated the experiment 9-10 times by maintaining aseptic condition and other cares to be taken while performing the experiment. Kindly give the solution for S. Aureus to get desired result. Thank You
I believe herbal drugs don't contain a specific active ingredient it has a combination of matters therefore the inhibition zone test might not be a great idea, yet researchers perform this test for pure active ingredients.
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