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Questions related to Biochemical Techniques
I'm looking for help measuring 25-hydroxycholesterol in cultured human macrophages. I would like to measure total cellular levels, including free 25-OHC and esterified 25-OHC. Does anyone have a...
05 May 2017 1,020 2 View
I want to calculate total number of Rosetta cells by using OD 600 , please explain.
05 May 2017 1,889 7 View
Hi guys. Does anyone have experience with the radiometric dye SNARF? I am trying to do a calibration step but I think that I am doing something wrong because in the end I cannot get meaningful pH...
05 May 2017 9,060 1 View
I'm having some troubles with my SDS-page and Western blot experiments. Usually I prepare protein samples diluting in Laemly buffer 5X with b-ME and boiling them for 5 minutes. When I perform the...
05 May 2017 5,470 6 View
Hi everyone, does anyone have a wb protocol on urine sample? Is it absolutly necessary to precipitate protein for example with TCA? Thanks in advance! Valeria
05 May 2017 7,001 2 View
On the company's advice we're using the Anions 2 kit from Analis, but can't make it work. We see three big peaks close to each other we would like to get further apart. Also even by running single...
01 May 2017 7,952 3 View
I would like to know the following: 1. what volume of phenol should be added to the unknown sample? 2 how much dilution should we do for unknown samples to be analyzed?
24 April 2017 7,547 2 View
I am curious, because in existing papers i can't seem to find a method of analysis that explains the big fluctuations of absorbency I am experiencing. I should be observing a linear decrease or no...
11 April 2017 9,074 1 View
I am having difficulties with visualizing my PCR products (bisulfite-converted cell-free DNAs) in agarose gel. I know my primers are working (tested them with control DNAs), my positive controls...
09 April 2017 3,925 3 View
Hi, I'm going to prepare PBAT libraries on CFSEdim and CFSEhi cells. I have doubts that CFSEdim and CFSEhi cells might have different DNA amount depending on their cell cycle phase. I couldn't...
04 April 2017 4,811 0 View
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04 April 2017 4,410 2 View
I am interested in the mechanisms of pH control in Streptomyces through Na+/H+ antiporters. Are there any good papers describing the control of these antiporters ?
21 March 2017 8,593 2 View
I have worked with curcin protein extracted from J. curcas on some cancer cell lines. so i need purified and sterilized protein.
15 March 2017 7,985 4 View
I am working Western Blot on rat cells. Thanks
14 March 2017 3,051 1 View
If yes, what concentration can be used?
08 March 2017 3,376 2 View
Hi , in this rx Sorbitol Dehydrogenase xylitol + NAD -----------------> D-xylulose + NADH2 - does anybody know if this reaction will still happen if i replaced the -...
06 March 2017 7,818 2 View
TRIZOL reagent was used for RNA extraction.
05 March 2017 8,087 3 View
I need a protocol to zymogram to beta-galactosidase and polygalacturonase. Can anyone help me?
03 March 2017 1,729 0 View
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03 March 2017 3,681 3 View
I have run out of 1x Plus Amplification Diluent (FP1135), what can be used to substitute for this reagent?
03 March 2017 880 1 View
I am charged with buying a new sonicator for my department. It will be used for bacterial lysis for protein purification.
03 March 2017 4,684 2 View
While doing transcription, the reaction was turbid with precipitant while incubation, but after I stopped the reaction and added my dye, I had negative results and no RNA appeared after running...
03 March 2017 1,433 1 View