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Questions related to Biochemical Techniques
We have being using these columns for a couple of months. Tend to re-use them according to salt (if it was used to desalt for DTT, for instance) and protein used. We do wash them with ddH2O and...
06 June 2015 5,870 3 View
Is there a way to detach proteins from microtubules (spindle microtubules) from a nuclear extract in vitro? I've tried to do this in vivo by treating living cells with nocodazole before...
05 May 2015 244 4 View
I have extracted Glucan Particles from Sacch.cerev. and tried to solubilize them in NaOH 0.05M, autoclaving the suspension. After that, the suspension becames brown and when I centrifuge to...
04 April 2015 8,843 1 View
Hi all, I have been having problems with my blots recently which I cannot explain logically so I have a few questions about the basics of Western blot and hoping your answers can shed some light...
19 March 2015 4,246 4 View
Kindly, brief me about the acetone ppt protocol.
17 March 2015 7,852 3 View
Does anybody has an experience with commercial kits for depletion of the most abundant proteins (IgG, albumin, etc.) for further analysis of low-abundant proteins? Thank you very much.
13 March 2015 8,778 5 View
I have problems about SDS Page and i have questions about it. 1- Is methanol / acetic acid fixation necessary before staining? 2- Should we use microwave oven to staining and destaining? Or,...
06 March 2015 7,597 7 View
I am looking to detect my transcript of interest in the brain ring gland complex in polysome fractions and I am wondering what is the minimum amount of dissections I will need to do. Thanks all!
05 March 2015 581 3 View
Hi all, I'm trying to calculate the theoretical time for a vesicle to sediment during centrifugation. I have (I think) all the information, but the numbers I'm getting out don't seem to make...
04 March 2015 7,456 3 View
i am looking for spectrophotometry estimation of 5HMF ?
03 March 2015 4,630 1 View
Hi Everyone, I am currently trying to standardize a protocol for Agrobacterium mediated gene transfer in plants. As a crucial step, induction of vir genes in Agro is mandatory and the composition...
03 March 2015 5,684 5 View
I confuse membrane filtration and gel filtration. Thank you.
03 March 2015 6,595 8 View
03 March 2015 9,816 6 View
How can (What are the techniques?) I detect a frameshift mutation in both the alleles of a gene induced by my CRISPR-Lentivirus? Please suggest. Syed
03 March 2015 10,098 2 View
03 March 2015 8,574 8 View
03 March 2015 1,337 4 View
03 March 2015 8,032 3 View
Now I'm using DNA probe modified with DNP(epitope) and DNP-antibody. I want to confirm that the DNA probe can be conjugated with DNP antibody thru DNP/DNP antibody binding. Do you know how I can...
02 February 2015 6,056 3 View
I am trying to replicate DNA stabilized silver nanocluster formation and visualize the various fluorescence. However, when I dissolve silver nitrate and mix it just with phosphate buffer (pH 6.7)...
02 February 2015 5,916 11 View
Hi everyone, The problem is that a chromatogram shows negative peaks after positive peak exiting, working with HPLC RI detector. The standard solution and the sample contain carbohydrates. MP: 25%...
02 February 2015 8,888 14 View
I am looking for a way to measure GSH in hemolysed blood samples. I have found a protocol from Browne and Armstrong (Methods in Molecular Biology, vol 108), using o-phthalaldehyde and...
02 February 2015 5,863 4 View
I will be driving these in the back of a U haul truck across several states. It is imperative that the samples stay frozen until they can be put into a -80 freezer. On line I have read 10 lbs of...
01 January 2015 5,111 6 View
I'm using a 30% polyacrylamide gel. I'm making a 6% gel with varied % concentrations of gelatin and varied concentration of trypsin. For my prelinimary experiment: 6% gel 0.1% gelatin 0.1%...
01 January 2015 10,008 9 View
This is according to the procedure in the leaflet attached here. Thank you.
01 January 2015 8,663 4 View