If yes, what concentration can be used?
totally different enzymes and substrates. I would not expect this to work.
ECL detects HRP activity on luminol
'luciferase' refers to a number of distinct enzymes with their own substrates e.g. firefly luciferase with D-luciferin
These are two different things. I have used luciferin for luciferase and ECL for Western, but never occurred to my mind to try to cross them, I would be very surprised if it works though.
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