Hi everyone,
does anyone have a wb protocol on urine sample? Is it absolutly necessary to precipitate protein for example with TCA?
Thanks in advance!
Valeria
If you prefer not to use TCA, you can probably concentrate the proteins using ultrafiltration.
Yes, we concentrate urine samples using centrifugal filter columns
I have to perform an ELISA assay on protein extracted from stool but I don't know which buffer is better. Daoes anyone have some experiences? Thank you very much!
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