I am working Western Blot on rat cells.
Thanks
We use anti-Clock from DSHB http://dshb.biology.uiowa.edu/
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
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Dear all, I have a question about standard population? Is Segi population outdated? I have a time series from 2000 onward. Regarding who 2000-2025 population from this link...
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Hello, During skeletal muscle contraction and during the actin–myosin ATPase cycle there is release of Pi and ADP. Can anyone tell me and ideally suggest a reading list of where they actually...
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Hello, I have no prior experience with growing cell line nor differentiation toward specific cell type. Currently I have C2C12 cell line which I need to grow and then differentiate it towards...
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Dear researchers, Google trends (GT) is very popular tool among SEO. However it is also use in public health. The lots of papers discuss results of searching google usig Trends. Hower, paper...
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Does anyone have experience for how long stock solution of tyrosinase can be stable to be applied again?
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Hello everyone, How to create a neural network with numerical values as input and an image as output? Can anyone give a hint/code for this scenario? Thank you in advance, Aleksandar Milicevic
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Hello, What software is most suitable for the automatic analysis of iPSCs neurons images (we are using ZEISS Cell Discoverer 7 for fluorescence imaging)? We are particularly interested in...
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Hi, all. I would like to know, how do you analyise data from the national registry that collects data on some diseases and conditions? In past, people used MS Access a lot. Is the R a proper...
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Hi, Can I use 37% formaldehyde (~10% methanol) to prepare 4% formaldehyde sol. in PBS for mice perfusion? How methanol can affect immunohistochemistry?
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
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I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
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