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Hi I am using a P/ACE MDQ Capillary Electrophoresis System from Beckman Coulter and trying to standardize the initial conditions to see the pI markers but the problem is that the baseline is not...
27 February 2017 5,482 7 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...
27 February 2017 3,787 2 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...
25 February 2017 740 22 View
02 February 2017 2,350 23 View
02 February 2017 5,822 2 View
02 February 2017 10,125 8 View
What effect would this have on the membrane? What is it recommended that I do?
02 February 2017 2,595 3 View
Hello everyone, I know it is a very basic question. Whenever I extract plasmid by alkaline lysis method and take nanodrop reading, it comes around 03-05ug/ul. Nanodrop peak is at exactly 260nm,...
02 February 2017 2,806 4 View
Does anyone have verified method for extraction of vitamin B from bread with sufficient extraction yield? I have used acidified water method...
02 February 2017 2,378 3 View
I require 100 uL of bovine ribonuclease III-A stock solution (5mg/mL). The product from Sigma-Aldrich is a very fluffy substance. I need to inactivate this twice at 100 degree C twice. Does anyone...
02 February 2017 2,496 3 View
01 February 2017 1,842 3 View
I need to know the types of RNA that you can analyze in an electrophoresis gel and the coefficient of sedimentation of Arabidopsis thaliana and CHO cells. I don't find the answer on the...
29 January 2017 1,439 8 View
01 January 2017 4,567 9 View
how to analyse contents in gel beads using HRSEM, EDS and FTIR, as wet gel bead is not preferred for these sensitive instruments?
01 January 2017 2,524 1 View
I am trying to see a G4 in an smFRET experiment. Although I am using 150mM NaCl and getting a FRET peak at 0.8 but the peak is diffused through 0 to 1 and not a sharp peak. Any one has any idea...
15 December 2016 2,646 7 View
Our slides coated with NMA with TBE always get cloudy after drying. We are using alkaline comet assay to evaluate sperm DNA fragmentation.
12 December 2016 9,530 6 View
I prepared blocking solution and RNA probe afresh but the same faint shift keeps on coming. To check whether the detection system is working I performed the detection system check with same RNA...
12 December 2016 5,112 2 View
12 December 2016 1,262 8 View
Hi everyone, Does anyone has a protocol that allows the extraction of F and G actin fraction in cells for the F:G actin ratio without using a kit? I have trouble to find that protocol but I know...
12 December 2016 9,287 1 View
12 December 2016 3,043 5 View
Hello. I'm recently researching for 'Maillard Reaction Products(MRPs)' made of sodium caseinate or whey protein isolates, and setting experiment for this. So I tried to dissolve MRPs ,which I...
07 December 2016 8,009 5 View
Copy from a paper : ‐Make 100 mM stock of: -‐ 25.5 ml 1 M Na2HPO4 -‐74.5 ml 1 M NaH2PO4 -‐pH to 6.4 with NaOH But can I adjust pH with NaOH?I thought that mix appropriate volumes of both...
10 October 2016 3,228 6 View
I only have a 3,000MWCO filter column to concentrate proteins in culture media. Is there a way I can alter the protocol to still include 80kD proteins?
08 August 2016 6,674 3 View
How can I concentrate protein from media to yield a higher concentration in reduced volume of another media?
03 August 2016 4,050 3 View