While doing transcription, the reaction was turbid with precipitant while incubation, but after I stopped the reaction and added my dye, I had negative results and no RNA appeared after running the gel. What might have gone wrong?
The most likely is the degradation of RNA, can you show the picture of gel ?
I'm trying to build .tpl files for the ribo-nucleotides to use them in MCCE program. I need to know the charges of the ribo-nucleotides (A,C,G,U) using amber force field. Is there a specific link...
03 April 2018 7,254 2 View
I'm currently studying the nervous system in details and I wanted an exact answer to the rate of transferring the signals. Moreover, if it is different in the white matter, does that affect the...
04 May 2015 9,577 4 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View