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Questions related to Biochemical Techniques
I am working on NFU3 protein, an scaffold Chloroplast target protein with CXXC motif. We believe that this protein involved in Fe-S clustering and their assembly. I am looking for the nice...
01 January 2015 9,279 5 View
I am using the published method to extract paraquat/diquat from potato. I used 10 g sample shake with 1:1 MeOH:0.1 N HCl for 10 min and heat in a water bath at 80 C for 15 min and shake more for...
01 January 2015 9,327 9 View
I am trying to regrow yeast that has been dried on filter paper. I have tried the following steps and have failed: I have dabbed the filter paper on ypd agar plates and had growth but I failed to...
01 January 2015 9,278 3 View
I performed MS method for peptide sequencing and now I am doing MS/MS for conformation but I am not getting exact spectra for same data. I kept my samples in 4°after MS analysis. I have seen the...
15 December 2014 763 5 View
12 December 2014 2,616 6 View
Whenever I am synthesizing peptide containing 6-20 amino acid sequence, the final yield was around 60% or below, can anyone suggest how to improve yield?
12 December 2014 9,576 6 View
So the enzyme activity is calculated as either increase of product concentration [P] or decrease in substrate concentration [S]. In practise, it's determined often using e.g. spectrophotometer as...
22 November 2014 513 3 View
I have been doing immunostaining of cultured cells. The current issue is that I don't see any positive stain. One thing that worries me is the fixation and permeabilization step. Is it possible...
22 November 2014 2,424 6 View
11 November 2014 5,586 3 View
Kindly get me a supplier who can deal with biochemical kits - lysosomal enzymes.
11 November 2014 3,823 3 View
Hello everyone, I am new to flow cytometry. In my experiment, I want to use single PI staining to detect cell cycle change and percentage of apoptotic cells. I used 70% ethanol to fix cells after...
11 November 2014 2,383 6 View
05 November 2014 3,642 5 View
As the RNAse H may not be 100% efficient at clevage, I want to seperate the cleaved from the uncleaved. I cannot seperate them on a gel due to their miniscule size difference of 15nt against a 1.9...
29 October 2014 1,255 5 View
Can anyone please tell me the effective concentration of trypsin used for trypsinization of H9c2 cells? I had used 1x trypsin from Sigma and got good results. But now I got 10x trypsin and it did...
28 October 2014 4,757 2 View
We have both paraffin- waxed tissue samples and blood from patients We would like to know what is the best method to use for the analysis of EBV?
23 October 2014 7,374 3 View
Any body know the difference between ficoll and percoll? Can I use percoll for isolation of the PBMC from whole blood and to use it?
15 October 2014 10,013 5 View
I have a procedure for analysis of total Glycyrrhic acid in Liquorice Dry Extract. But I want to evaluate 18β-glycyrrhizic acid. Can anybody tell me what is the percentage content of...
11 October 2014 4,044 2 View
I wanted to use proteinase K to treat conditioned media in order to determine if a protein factor is responsible for the effect seen when CM is used on cells. I have come across various protocols...
10 October 2014 862 4 View
I tried to replicate my experiment where all my bands were perfectly aligned and twice now each band has a different angle. Why?
10 October 2014 530 5 View
I am at my wit's end with this and do not know where else to turn. I am a new Lab Technician in this lab and this PCR has become a point of serious stress in becoming a competent and trusted...
10 October 2014 2,117 9 View
I developed a protocol for homogenizing lung tissue using 1mm glass beads and a BioSpec MiniBead Beater-16 at my previous institution but I have not been able to locate a bead homogenizer at my...
10 October 2014 3,989 0 View
Hello, I am wondering if it is possible to get intact RNA -good for microarray/qPCR gene expression assays- from pork or beef carcass a couple of days post-slaughter. Any information of...
10 October 2014 8,242 11 View
Yellow Western Experts! My last blot came out looking like this wonderful picture. It is absolutely maddening, I dont know whats causing this crazy background. I do the exact same thing and...
10 October 2014 5,761 9 View
Hi, Iam using MC3T3 E1 cells for my experiment. For an experiment I was growing the cells for 10 days. Daily I used to collect the cells for western. On the 5th day, after I added the lysis...
10 October 2014 8,223 3 View