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Questions related to Biochemical Techniques
Can someone help with how I can set up experiment to validate PCR primers using stock cDNA samples? What type of primer dilution series (concentrations) should I test? Thanks!
04 May 2016 6,493 3 View
Pyrite bioleaching solutions have lower pH values than pyrite chemical control solutions. But it is reverse in chalcopyrite solutions. What can be the reason?
03 March 2016 7,656 3 View
I'm doing research on bio-degradation of coffee husk/pulp for biogas.
02 February 2016 7,269 2 View
Carrots are extracted with 80% alcohol
02 February 2016 1,666 2 View
We need to observe a compound (curcumin) in the footpad of a mouse. Solvents used for histopathology are formaldehyde and nitric acid. In addition, we have to decalcify the bone in order to do the...
02 February 2016 8,846 4 View
I am wondering what the diffusion coefficients of PEG in the range of 200-1000 MW in water, as well as in epithelial tissues. I would also Like to know what the partition coefficients would be...
06 January 2016 1,505 2 View
I have recently read articles about the biochemical characterisation of proteins, and see people use dimer stability, structural stability and thermal stability, I wonder what's the differences...
01 January 2016 1,832 6 View
01 January 2016 3,000 2 View
I have bought Sigma Isoproterenol Hydrochloride (I6504). I have dissolved it in KRBH buffer (pH-7.4) to make it 50 mM stock and stored @ -20. I used 10 micromolar conc. to treat adipocytes for 1-2...
30 November 2015 3,404 12 View
I want to use RNase H from Invitrogen, but E.coli polymerase I and E.coli ligase from NEB. Can I use the Second Strand Synthesis Reaction Buffer from NEB? Also, NEB states that their System is...
24 November 2015 3,007 2 View
Hi, I've done western blot on LV tissues and I want to prepare whole cell extract for west blot analysis using frozen gonads and uterine tissues as positive controls for my proteins of...
16 November 2015 6,685 2 View
11 November 2015 6,999 13 View
11 November 2015 6,246 2 View
11 November 2015 3,517 2 View
I use Aebi method in the assay of catalase, but i have a problem : the absorbency is unstable which mean that the numbers increase and decrease rapidly.
11 November 2015 3,060 34 View
For example using Ripa buffer? Or will the immunoglobulins fall apart?
03 November 2015 4,212 1 View
02 November 2015 7,977 33 View
I would like to determine the purity of my plasma membrane extract from plant material. Can anyone tell me how to determine e.g. vanadate-sensitive ATPase or other markers? Or would you recommend...
10 October 2015 1,511 2 View
Hi guys! I have to store plasma after centrifugation from whole blood. The problem is that I don't have a -80°C freezer and I want to extract RNA from plasma at different time after storage. How...
09 September 2015 671 5 View
Hello everyone, I'm trying to find a way to isolate PBMC from very small blood samples (approx. 250µL, but sometimes less). I already tried using a RBC lysis buffer, centrifugation and washing,...
27 August 2015 9,799 4 View
if i want to detect Prpsc through WB, then i need to make brain homogenate and sonicate it first and centrifuged at 10K x g, but whether i will put PK after centrifugation or before? i personally...
13 August 2015 1,132 2 View
08 August 2015 6,026 5 View
08 August 2015 8,634 2 View
We have being using these columns for a couple of months. Tend to re-use them according to salt (if it was used to desalt for DTT, for instance) and protein used. We do wash them with ddH2O and...
18 June 2015 9,219 3 View