I mean using the same DNA for the same pcr conditions you get varying result even when ran at the same time. That is some faint bands while some are bright enough.
We are going to need some more details here.
First, are you certain you are seeing multiple PCR products and not just primer dimers?
Two, do you expect to see only one band?
Is your gene of interest multi-copy in the genome? That can lead to multiple bands.
Are there two or more different size alleles of your gene of interest? Insertion/deletion alleles will give different size products and band intensities.
Finally, what do your controls look like?
What I am seeing is not primer dimer because I have used the result to run obtained from good bands to run restriction digestion which gave very good results.
yes I expect to see only one band.
please see here: https://www.neb.com/tools-and-resources/troubleshooting-guides/pcr-troubleshooting-guide
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